Marchf8^tm1.1Lkle
Targeted Allele Detail

Summary

• Symbol: Marchf8^tm1.1Lkle
• Name: membrane associated ring-CH-type finger 8; targeted mutation 1.1 Ludger Klein
• MGI ID: MGI:5819100
• Gene: Marchf8 Location: Chr6:116314985-116386501 bp, + strand Genetic Position: Chr6, 53.78 cM, cytoband F1
• Alliance: Marchf8^tm1.1Lkle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236611
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP flanked neomycin selection cassette replaced exons 5 and 6, which encode the catalytically active site in the RING variant (RINGv) domain and the TM domain, respectively. Mice carrying tjhe targeted allele were mated to a CAG-Cre deleter mouse line to remove the neomycin cassette in the final allele. (J:236611)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Marchf8 Mutation: 37 strains or lines available

References

• Original: J:236611 von Rohrscheidt J, et al., Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83. J Exp Med. 2016 Aug 22;213(9):1685-94
• All: 1 reference(s)