Gsk3a^tm1.2Svij
Targeted Allele Detail

Summary

• Symbol: Gsk3a^tm1.2Svij
• Name: glycogen synthase kinase 3 alpha; targeted mutation 1.2, Srinivasan Vijayaraghavan
• MGI ID: MGI:5752934
• Gene: Gsk3a Location: Chr7:24927683-24937276 bp, - strand Genetic Position: Chr7, 13.73 cM
• Alliance: Gsk3a^tm1.2Svij page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221688
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was introduced upstream of exon 2 and an FRT flanked neo cassette and loxP site were introduced downstream of exon 4 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 2-4. Western blot analysis confirmed the absence of protein expression in testis from homozygous mice. (J:221688)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gsk3a Mutation: 18 strains or lines available

References

• Original: J:221688 Bhattacharjee R, et al., Targeted disruption of glycogen synthase kinase 3A (GSK3A) in mice affects sperm motility resulting in male infertility. Biol Reprod. 2015 Mar;92(3):65
• All: 2 reference(s)