Fosb^tm1.1Yun
Targeted Allele Detail

Summary

• Symbol: Fosb^tm1.1Yun
• Name: FBJ osteosarcoma oncogene B; targeted mutation 1.1, Yusaku Nakabeppu
• MGI ID: MGI:5634956
• Synonyms: Fosb^d
• Gene: Fosb Location: Chr7:19036621-19043970 bp, - strand Genetic Position: Chr7, 9.56 cM, cytoband A2-B1
• Alliance: Fosb^tm1.1Yun page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220448
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A floxed neo cassette was inserted in intron 3 and the sequence encoding the alternative splicing donor site for the exonic intron in exon 4 was replaced with tandem stop codons (GTG, AGA to TAG, TGA) via homologous recombination. This prevents the expression of the short transcript but allows expression of the longer transcript that encodes two isoforms (deltaFosb and delta2deltaFosb). Cre-mediated recombination removed the neo cassette. (J:220449)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fosb Mutation: 18 strains or lines available

References

• Original: J:220449 Ohnishi YN, et al., Antagonistic regulation of cell-matrix adhesion by FosB and DeltaFosB/Delta2DeltaFosB encoded by alternatively spliced forms of fosB transcripts. Mol Biol Cell. 2008 Nov;19(11):4717-29
• All: 4 reference(s)