Poli^tm1.1Lgh
Targeted Allele Detail

Summary

• Symbol: Poli^tm1.1Lgh
• Name: polymerase (DNA directed), iota; targeted mutation 1.1, Gang-Hong Lee
• MGI ID: MGI:5582769
• Gene: Poli Location: Chr18:70641751-70663691 bp, - strand Genetic Position: Chr18, 44.48 cM, cytoband E2
• Alliance: Poli^tm1.1Lgh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212610
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 5. An FRT-flanked neomycin resistance cassette with a 3 loxP site was inserted downstream of exon 7. Cre-mediated recombination removed the selection cassette, exon 6 and exon 7. These exons encode the conserved motif IV and V (helix-hairpin-helix region for DNA binding). Western blot analysis confirmed the absence of full-length and truncated version of the protein in testes. (J:212610)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Poli Mutation: 45 strains or lines available

References

• Original: J:212610 Iguchi M, et al., The error-prone DNA polymerase iota provides quantitative resistance to lung tumorigenesis and mutagenesis in mice. Oncogene. 2014 Jul 3;33(27):3612-7
• All: 1 reference(s)