Cyp11b2^{tm1.1(cre)Brlt}
Targeted Allele Detail

Summary

• Symbol: Cyp11b2^{tm1.1(cre)Brlt}
• Name: cytochrome P450, family 11, subfamily b, polypeptide 2; targeted mutation 1.1, David T Breault
• MGI ID: MGI:5543601
• Synonyms: AS^Cre
• Gene: Cyp11b2 Location: Chr15:74722859-74728167 bp, - strand Genetic Position: Chr15, 34.29 cM
• Alliance: Cyp11b2^{tm1.1(cre)Brlt} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204614
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Cyp11b2^{tm1.1(cre)Brlt} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cyp11b2 (MGI:88584)

• Mutation details: A targeting construct with cre recombinase and an Frt-flanked neo cassette was inserted by homologous recombination into the Cyp11b2 (aldosterone synthase, AS) locus, replacing most of the coding region, retaining only exon 1, intron 1 and the first part of exon 2. Germline animals were crossed to Flpe deleter mice to remove the neo cassette. Mice homozygous for the allele are effectrively null for Cyp11b2 function. (J:204614)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cyp11b2 (mouse) Summary of all recombinase alleles driven by Cyp11b2. Alliance mouse Cyp11b2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp11b2 Mutation: 40 strains or lines available

References

• Original: J:204614 Freedman BD, et al., Adrenocortical zonation results from lineage conversion of differentiated zona glomerulosa cells. Dev Cell. 2013 Sep 30;26(6):666-73
• All: 15 reference(s)