Nr6a1^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nr6a1^{tm1d(EUCOMM)Wtsi}
• Name: nuclear receptor subfamily 6, group A, member 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5532717
• Synonyms: Gcnf^delta
• Gene: Nr6a1 Location: Chr2:38613381-38816473 bp, - strand Genetic Position: Chr2, 24.42 cM
• Alliance: Nr6a1^{tm1d(EUCOMM)Wtsi} page
• IMPC: Nr6a1 gene page

Morphological defects in E9.5 Nr6a1^{tm1d(EUCOMM)Wtsi}/Nr6a1^{tm1d(EUCOMM)Wtsi} embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0105_6_D03, EPD0105_6_D04
• Germline Transmission: Earliest citation of germline transmission: J:204241
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 38632589 of Chromosome 2 upstream of the critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 38633441. The critical exon 7 is thus flanked by loxP sites. Flp-mediated recombination removed the reporter and selection cassette. Cre-mediated recombination removed exon 7. (J:204241)

Generation of the Nr6a1^tm1a(EUCOMM)Wtsi, Nr6a1^tm1c(EUCOMM)Wtsi, and Nr6a1^tm1d(EUCOMM)Wtsi alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nr6a1 Mutation: 145 strains or lines available

Notes

Cell line EPD0105_6_D03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:204241

References

• Original: J:204241 Okumura LM, et al., The ligand binding domain of GCNF is not required for repression of pluripotency genes in mouse fetal ovarian germ cells. PLoS One. 2013;8(6):e66062
• All: 1 reference(s)