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| Caption | Targeted disruption of Nr6a1 (Gcnf). (A) Genomic structure of wild-type Nr6a1 locus. Exons 1-12 are numbered and shown as gray vertical lines or boxes. The exons encoding the DNA binding domain (previously disrupted by Chung, et al.) and ligand binding domain (disrupted here) are indicated. (B) Gene-trap allele Nr6a1tm1a(EUCOMM)Wtsi (Gcnfgt) present in ES cells obtained from EUCOMM (only exons 6-8 are shown). Gene-trap cassette contains a splice acceptor (SA) and internal ribosome entry site (IRES) upstream of a lacZ reporter gene followed by a polyadenylation (pA) signal. Selection cassette consists of a neomycin resistance gene (neo) driven by an autonomous promoter (hBactP) and pA signal. Both cassettes are flanked by FLP recombination target (FRT) sites. (C) After FLP-mediated recombination in vivo, the gene-trap and neomycin-resistance cassettes are removed, leaving a true conditional knockout allele, referred to as Nr6a1tm1c(EUCOMM)Wtsi (Gcnffl), with loxP sites flanking exon 7. (D) Cre-mediated recombination between the loxP sites, which deletes exon 7 results in the Nr6a1tm1d(EUCOMM)Wtsi (Gcnfdelta). All structures drawn to scale; scale bars for (A) and (B-D) are shown. | ||||||||||
| Copyright | This image is from Okumura LM, PLoS One 2013;8(6):e66062, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:204241 | ||||||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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