Ephb6/Trpv6^tm2.2Mfre
Targeted Allele Detail

Summary

• Symbol: Ephb6/Trpv6^tm2.2Mfre
• Name: Eph receptor B6; targeted mutation 2.2, Marc Freichel
• MGI ID: MGI:5498760
• Synonyms: Trpv6^L2F1
• Gene: Ephb6 Location: Chr6:41582416-41597443 bp, + strand Genetic Position: Chr6, 19.77 cM
• Alliance: Ephb6/Trpv6^tm2.2Mfre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185996
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: In the Trpv6 gene a loxP site was inserted into intron 12 and an FRT site flanked neomycin resistance gene cassette with a 3' loxP site was inserted downstream of the last exon (15) into intron 16 of the Ephb6 gene on the opposite strand. Subsequent flp-mediated recombination removed the neo cassette, leaving a conditional-ready allele with exons 13-15 of Trpv6 as well as exons 17 and 18 of Ephb6 floxed. (J:185996)

Generation of the Ephb6/Trpv6^tm2.2Mfre allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ephb6 Mutation: 54 strains or lines available

References

• Original: J:185996 Weissgerber P, et al., Male fertility depends on Ca(2)+ absorption by TRPV6 in epididymal epithelia. Sci Signal. 2011;4(171):ra27
• All: 3 reference(s)