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| Caption | Strategy to flank exons 13, 14, and 15, which encode the pore region, part of the fifth and the entire sixth transmembrane-spanning domains, and the cytosolic C terminus of TRPV6, with loxP sites. a, the wild-type Trpv6+ allele and targeting construct. Translated exons (not in scale) are shown as filled boxes. Exons 13, 14, and 15 are flanked by loxP sites (filled triangles). An FRT site (gray triangles)-flanked PGK-neor cassette is located upstream of the second loxP site. b, Flp-mediated excision of the PGK-neor cassette produces the Ephb6/Trpv6tm2.2Mfre allele that has exons 13, 14, and 15 flanked by loxP sites. B, BamHI; X, XhoI. Probes and sizes of genomic DNA fragments as expected by Southern blots are indicated. HSVtk, herpes simplex virus thymidin kinase. | ||||||
| Copyright | This image is from Weissgerber P, J Biol Chem 2012 May 25;287(22):17930-41 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:185629 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 03/18/2025 MGI 6.24 |
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