Gt(ROSA)26Sor^{tm1(CAG-PIK3CA*H1047R,-EGFP)Balj}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-PIK3CA*H1047R,-EGFP)Balj}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Mohamed Bentires-Alj
• MGI ID: MGI:5052133
• Synonyms: PIK3CA H1047R
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-PIK3CA*H1047R,-EGFP)Balj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173655
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: BALB/c

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, RMCE-ready, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-PIK3CA*H1047R,-EGFP)Balj} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PIK3CA (5290)

  Pik3ca

  (MGI:1206581)

• Mutation details: RMCE inserted a vector, which contained a CAG promoter driving expression of an HA-tagged human cDNA with an A to G transition that result in the amino acid arginine for histidine at position 1047 (H1047R) preceded by a floxed stop cassette and followed by an IRES2-EGFP, into the locus. (J:173655)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

References

• Original: J:173655 Meyer DS, et al., Luminal Expression of PIK3CA Mutant H1047R in the Mammary Gland Induces Heterogeneous Tumors. Cancer Res. 2011 Jul 1;71(13):4344-51
• All: 4 reference(s)