Actb^tm1(INSR)Dac
Targeted Allele Detail

Summary

• Symbol: Actb^tm1(INSR)Dac
• Name: actin, beta; targeted mutation 1, Dominico Accili
• MGI ID: MGI:4831148
• Synonyms: beta/INSR
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^tm1(INSR)Dac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91350
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, No functional change)
• Mutation: Insertion
• Actb^tm1(INSR)Dac expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  INSR (3643)

  Insr	(MGI:96575)

• Mutation details: An IRES, floxed neomycin selection cassette with a polyadenylation signal, and a human insulin receptor cDNA were inserted downstream of the stop codon of the endogenous gene. Expression of the inserted INSR cDNA requires cre excision of the neomycin cassette. (J:91350)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 51 strains or lines available

References

• Original: J:91350 Okamoto H, et al., Transgenic rescue of insulin receptor-deficient mice. J Clin Invest. 2004 Jul;114(2):214-23
• All: 3 reference(s)