Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir21)Fjsl}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir21)Fjsl}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Frank J Slack
• MGI ID: MGI:4830734
• Synonyms: Gt(ROSA)26Sor^{tm1(tetO-Mir21)Fjsl}, Gt(ROSA)26Sor^{tm1(tetO-Mirn21)Fjsl}, mir-21^LSL-Tetoff
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir21)Fjsl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163919
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6J x SJL/J)F2

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Inserted expressed sequence, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir21)Fjsl} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mir21a (MGI:2676885)

• Mutation details: A construct was inserted into the locus that contained the following elements (5' to 3'): the tetracycline transactivator (tTA), two poly-adenylation sequences, the tetracycline operator with minimal CMV promoter, a floxed neo cassette with 4 poly-adenylation sequences, the mouse transcript, and a poly-adenylation sequence. The locus promoter drives expression of tTA while the tet operator drives expression of the transcript only after cre mediated removal of the floxed neo-STOP cassette that can be repressed by doxycycline treatment. (J:163919)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

References

• Original: J:163919 Medina PP, et al., OncomiR addiction in an in vivo model of microRNA-21-induced pre-B-cell lymphoma. Nature. 2010 Sep 2;467(7311):86-90
• All: 2 reference(s)