Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir21)Fjsl}
• Allele Name: targeted mutation 1, Frank J Slack
• Allele ID: MGI:4830734
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(tTA,tetO-Mir21)Fjsl/Gt(ROSA)26Sor^+ Tg(Nes-cre)1Wmz/0	involves: C57BL/6 * C57BL/6J * SJL/J	MGI:4830769

Genotype
MGI:4830769

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir21)Fjsl}/Gt(ROSA)26Sor⁺; Tg(Nes-cre)1Wmz/0
• Genetic Background: involves: C57BL/6 * C57BL/6J * SJL/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

enlarged spleen ( J:163919 )

• 8-fold in severe cases after doxycycline withdrawal

mortality/aging

premature death ( J:163919 )

• mice not fed doxycycline exhibit die before 3 months of age unlike wild-type mice

• however, mice fed doxycycline are alive at 3 months of age

immune system

abnormal thymus morphology ( J:163919 )

• after doxycycline withdrawal, thymus structure is altered by invasion of neoplastic cells unlike in similarly treated wild-type mice

abnormal thymus corticomedullary boundary morphology ( J:163919 )

• after doxycycline withdrawal

enlarged thymus ( J:163919 )

• 20-fold in severe cases after doxycycline withdrawal

decreased leukocyte cell number ( J:163919 )

• in 4 of 7 mice at 3 months

abnormal spleen morphology ( J:163919 )

• after doxycycline withdrawal, spleen abnormalities are due to malignant invasion of red pulp unlike in similarly treated wild-type mice

enlarged spleen ( J:163919 )

• 8-fold in severe cases after doxycycline withdrawal

intermingled spleen red and white pulp ( J:163919 )

• after doxycycline withdrawal

enlarged lymph nodes ( J:163919 )

• two months after doxycycline withdrawal and in mice not fed doxycycline

neoplasm

abnormal tumor morphology ( J:163919 )

• mice fed doxycycline after a period of not consuming it exhibit regression of lymphomas associated with increased apoptosis of tumor cells and decreased cell proliferation compared with untreated mice

increased tumor incidence ( J:163919 )

• after doxycycline withdrawal, mice exhibit malignant invasion in the spleen, thymus, peripheral blood, and other organs, especially the liver and less frequently the kidney, unlike in similarly treated wild-type mice

• however, mice fed doxycycline exhibit remission of tumors and tumor-associated pathologies

increased lymphoma incidence ( J:163919 )

• mice not fed doxycycline exhibit external signs of lymphoma (lymphadenopathy and/or paresis of rear limbs) unlike wild-type mice

• however, mice fed doxycycline do not exhibit pathologies associated with lymphomas

behavior/neurological

hunched posture ( J:163919 )

• two months after doxycycline withdrawal

hindlimb paresis ( J:163919 )

• paresis in the hindlimbs two months after doxycycline withdrawal

skeleton

abnormal bone marrow morphology ( J:163919 )

• after doxycycline withdrawal, mice exhibit extended bone marrow in the thoracic and lumbar vertebrae, femur, sternum, and hard palate causing paresis, bone fractures, and invasion of surrounding tissue unlike similarly treated wild-type mice

respiratory system

respiratory distress ( J:163919 )

• two months after doxycycline withdrawal

hematopoietic system

abnormal thymus morphology ( J:163919 )

• after doxycycline withdrawal, thymus structure is altered by invasion of neoplastic cells unlike in similarly treated wild-type mice

abnormal thymus corticomedullary boundary morphology ( J:163919 )

• after doxycycline withdrawal

enlarged thymus ( J:163919 )

• 20-fold in severe cases after doxycycline withdrawal

anemia ( J:163919 )

• at 3 months

• however, mice treated with doxycyline do not exhibit anemia

decreased erythrocyte cell number ( J:163919 )

• at 3 months

decreased hemoglobin content ( J:163919 )

• at 3 months

decreased leukocyte cell number ( J:163919 )

• in 4 of 7 mice at 3 months

abnormal spleen morphology ( J:163919 )

• after doxycycline withdrawal, spleen abnormalities are due to malignant invasion of red pulp unlike in similarly treated wild-type mice

enlarged spleen ( J:163919 )

• 8-fold in severe cases after doxycycline withdrawal

intermingled spleen red and white pulp ( J:163919 )

• after doxycycline withdrawal

integument

ruffled hair ( J:163919 )

• two months after doxycycline withdrawal

endocrine/exocrine glands

abnormal thymus morphology ( J:163919 )

• after doxycycline withdrawal, thymus structure is altered by invasion of neoplastic cells unlike in similarly treated wild-type mice

abnormal thymus corticomedullary boundary morphology ( J:163919 )

• after doxycycline withdrawal

enlarged thymus ( J:163919 )

• 20-fold in severe cases after doxycycline withdrawal