Slc26a1^tm1Mark
Targeted Allele Detail

Summary

• Symbol: Slc26a1^tm1Mark
• Name: solute carrier family 26 (sulfate transporter), member 1; targeted mutation 1, Daniel Markovich
• MGI ID: MGI:4440647
• Synonyms: Sat1^-
• Gene: Slc26a1 Location: Chr5:108817744-108823435 bp, - strand Genetic Position: Chr5, 53.24 cM
• Alliance: Slc26a1^tm1Mark page

Urolithiasis in Slc26a1^tm1Mark/Slc26a1^tm1Mark mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158567
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Exon 3 (containing the start codon) was replaced with a neo cassette by homologous recombination. Northern blot analysis of RNA from liver, kidney and ileum showed absence of full length RNA in homozygotes. Western blot analysis of protein from the intestines confirmed absence of the wild-type protein. The mutation was designed to avoid affecting the surrounding gene, Idua, absence of an effect was confirmed by RT-PCR and enzyme activity analyses. (J:158567)

Generation of the Slc26a1^tm1Mark allele

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc26a1 Mutation: 23 strains or lines available

References

• Original: J:158567 Dawson PA, et al., Urolithiasis and hepatotoxicity are linked to the anion transporter Sat1 in mice. J Clin Invest. 2010 Mar;120(3):706-12
• All: 6 reference(s)