Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli
Transgene Detail

Summary

• Symbol: Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli
• Name: transgene insertion 14, Jack A Elias
• MGI ID: MGI:3836020
• Synonyms: tet-O-CMV-IFNgamma-hGH, TG CC10-rtTA-IFNgamma
• Transgene: Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli Location: unknown
• Alliance: Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli page

Transgene origin

• Strain of Origin: (CBA x C57BL/6)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ifng (MGI:107656)

• Mutation details: The CC10-rtTA-hGH construct contains the CC10 promoter, the reverse tetracycline transactivator (rtTA), and human growth hormone (hGH) intronic and polyadenylation sequences. The tet-O-CMV-IFNgamma-hGH construct contains a polymeric tetracycline operator (tet-O), minimal CMV promoter, murine IFNgamma cDNA, and hGH intronic and polyadenylation signals. Both constructs were coinjected into fertilized oocytes, and dual positive founder animals were obtained. In the presence of doxycycline (dox), rtTA is able to bind in trans to the tet-O, and the VP-16 transactivator activates LIF gene transcription. In the absence of dox, rtTA binding occurs at very low levels, and only low level gene transcription is noted. Transgenic lines 15 and 21 were also created. (J:66079)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:66079 Wang Z, et al., Interferon gamma induction of pulmonary emphysema in the adult murine lung. J Exp Med. 2000 Dec 4;192(11):1587-600
• All: 5 reference(s)