Sox10^tm5Weg
Targeted Allele Detail

Summary

• Symbol: Sox10^tm5Weg
• Name: SRY (sex determining region Y)-box 10; targeted mutation 5, Michael Wegner
• MGI ID: MGI:3723291
• Synonyms: Sox10^deltaK2
• Gene: Sox10 Location: Chr15:79039113-79048690 bp, - strand Genetic Position: Chr15, 37.7 cM
• Alliance: Sox10^tm5Weg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124679
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was constructed that contains a Sox10 open reading that encodes for a deletion of amino acids 233-306, followed by a loxP flanked neo cassette. The region deleted has been implicated in the transactivation potential of this transcription factor. The vector replaced the entire open reading frame of Sox10 by deleting exons 3 through 5. The neo cassette was removed via in vivo Cre mediated recombination. Quantitative RT-PCR on mid-stage embryos indicated that mRNA levels of the mutated gene were similar to the wild-type gene. (J:124679)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox10 Mutation: 33 strains or lines available

References

• Original: J:124679 Schreiner S, et al., Hypomorphic Sox10 alleles reveal novel protein functions and unravel developmental differences in glial lineages. Development. 2007 Sep;134(18):3271-81
• All: 3 reference(s)