Dnmt3b^tm1Jae
Targeted Allele Detail

Summary

• Symbol: Dnmt3b^tm1Jae
• Name: DNA methyltransferase 3B; targeted mutation 1, Rudolf Jaenisch
• MGI ID: MGI:3623148
• Synonyms: Dnmt3b^2lox, Dnmt3b^L
• Gene: Dnmt3b Location: Chr2:153491370-153529650 bp, + strand Genetic Position: Chr2, 75.79 cM, cytoband A2-A3
• Alliance: Dnmt3b^tm1Jae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:107386
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a frt-flanked PGK-neo and a loxP site into intron 15 and a second loxP site into intron 19. The floxed exons encode the catalytic domains. The neo was removed via FLP mediated recombination. Western blot confirmed that this is a silent mutation. (J:107386)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dnmt3b Mutation: 50 strains or lines available

References

• Original: J:107386 Lin H, et al., Suppression of intestinal neoplasia by deletion of Dnmt3b. Mol Cell Biol. 2006 Apr;26(8):2976-83
• All: 12 reference(s)