Ly6a^{tm2(RUNX1)Grau}
Targeted Allele Detail

Summary

• Symbol: Ly6a^{tm2(RUNX1)Grau}
• Name: lymphocyte antigen 6 family member A; targeted mutation 2, Timothy A Graubert
• MGI ID: MGI:3510673
• Synonyms: Ly6a^tm2Grau, Sca1^AE, Sca1^{AML1-ETO ires EGFP}
• Gene: Ly6a Location: Chr15:74866726-74869880 bp, - strand Genetic Position: Chr15, 34.29 cM
• Alliance: Ly6a^{tm2(RUNX1)Grau} page

Morophologic analysis of myeloproliferation disorder in Ly6a^{tm2(RUNX1)Grau}/Ly6a⁺ mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93408
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert human RUNX1 (CBFA2T1), IRES-EGFP and floxed PGK-neo into exon 2 of the endogenous gene. Expression of the inserted mRNA was detectable in peripheral blood leukocytes from heterozygous animals by RT-PCR. Flow cytometry indicated GFP was highly expressed in peripheral blood and bone marrow cells. (J:93408)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ly6a Mutation: 22 strains or lines available

References

• Original: J:93408 Fenske TS, et al., Stem cell expression of the AML1/ETO fusion protein induces a myeloproliferative disorder in mice. Proc Natl Acad Sci U S A. 2004 Nov 19;101(42):15184-9
• All: 1 reference(s)