Igh^tm5Cgn
Targeted Allele Detail

Summary

• Symbol: Igh^tm5Cgn
• Name: immunoglobulin heavy chain complex; targeted mutation 5, University of Cologne
• MGI ID: MGI:3037892
• Synonyms: LMP2A^flSTOP
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88472
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: BALB/c

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: To conditionally express the Epstein-Barr virus protein, LMP2A, from either the endogenous weak DQ52 promoter or an inserted strong Igh-V 186.2 promoter, sequence encoding LMP2A was inserted downstream of a loxP-flanked stop cassette. Floxed Igh-V 186.2 promoter sequence was included in the targeting vector between the DQ52 promoter and the floxed stop cassette (which contained frt-flanked neo followed by stop codons). Germline excision of the floxed Igh-V promoter and the stop cassette led to the production of Igh^tm5.1Cgn. (J:88472)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 43 strains or lines available

References

• Original: J:88472 Casola S, et al., B cell receptor signal strength determines B cell fate. Nat Immunol. 2004 Mar;5(3):317-27
• All: 3 reference(s)