Phenotypes associated with this allele

• Allele Symbol: Igh^tm5Cgn
• Allele Name: targeted mutation 5, University of Cologne
• Allele ID: MGI:3037892
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Igh^tm5Cgn/Igh^tm5Cgn	involves: BALB/c	MGI:3037900
cn2	Igh^tm5Cgn/Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn/0	C.Cg-Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn	MGI:3037901

Genotype
MGI:3037900

hm1

• Allelic Composition: Igh^tm5Cgn/Igh^tm5Cgn
• Genetic Background: involves: BALB/c

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

arrested B cell differentiation ( J:88472 )

• homozygotes display an arrest of B cell development at the B220⁺CD43⁺ pro-B cell stage

absent B cells ( J:88472 )

• homozygotes lack peripheral B cells

immune system

arrested B cell differentiation ( J:88472 )

• homozygotes display an arrest of B cell development at the B220⁺CD43⁺ pro-B cell stage

absent B cells ( J:88472 )

• homozygotes lack peripheral B cells

Genotype
MGI:3037901

cn2

• Allelic Composition: Igh^tm5Cgn/Igh^tm5Cgn; Tg(CD19-cre/ERT2)1Cgn/0
• Genetic Background: C.Cg-Igh^tm5Cgn Tg(CD19-cre/ERT2)1Cgn

hematopoietic system

abnormal B cell differentiation ( J:88472 )

• B cell development is almost exclusively restricted to the generation of B-1 cells

increased pro-B cell number ( J:88472 )

• mutant mice display a higher percentage of pro-B cells (CD19⁺CD43⁺) in the bone marrow relative to wild-type controls

increased B cell number ( J:88472 )

• mutant mice display an increase in the number of B cells in spleen (4-fold), lymph nodes (2-fold) and peritoneal cavity (2-fold) relative to age-matched wild-type controls

abnormal mature B cell morphology ( J:88472 )

• >90% of peripheral mutant B cells exhibit a B-1 cell phenotype (CD24^intCD38^hi)

• no follicular and marginal zone B cells are identified, as most splenic B cells are CD5^intCD21^loCD23^lo

absent follicular B cells ( J:88472 )

absent marginal zone B cells ( J:88472 )

immune system

abnormal Peyer's patch germinal center morphology ( J:88472 )

• non-immunized mutant mice exhibit occasional spontaneous germinal center formation in Peyer's patches

abnormal B cell differentiation ( J:88472 )

• B cell development is almost exclusively restricted to the generation of B-1 cells

increased pro-B cell number ( J:88472 )

• mutant mice display a higher percentage of pro-B cells (CD19⁺CD43⁺) in the bone marrow relative to wild-type controls

increased B cell number ( J:88472 )

• mutant mice display an increase in the number of B cells in spleen (4-fold), lymph nodes (2-fold) and peritoneal cavity (2-fold) relative to age-matched wild-type controls

abnormal mature B cell morphology ( J:88472 )

• >90% of peripheral mutant B cells exhibit a B-1 cell phenotype (CD24^intCD38^hi)

• no follicular and marginal zone B cells are identified, as most splenic B cells are CD5^intCD21^loCD23^lo

absent follicular B cells ( J:88472 )

absent marginal zone B cells ( J:88472 )

digestive/alimentary system

abnormal Peyer's patch germinal center morphology ( J:88472 )

• non-immunized mutant mice exhibit occasional spontaneous germinal center formation in Peyer's patches