Lipe^tm1Rze
Targeted Allele Detail

Summary

• Symbol: Lipe^tm1Rze
• Name: lipase, hormone sensitive; targeted mutation 1, Rudolf Zechner
• MGI ID: MGI:2388334
• Synonyms: HSL^-, HSL-ko
• Gene: Lipe Location: Chr7:25078952-25097911 bp, - strand Genetic Position: Chr7, 13.78 cM, cytoband A3
• Alliance: Lipe^tm1Rze page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74644
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin selection cassette was inserted in intron 1 and an additional loxP site was inserted 3' to exon 7. The resulting targeted ES cells were subjected to transient cre expression. Exons 2 to 7 as well as neomycin selection cassette were deleted. In addition to this allele, transient expression provided recombinant ES cells in which only the selection cassette was deleted. These cells were used to produce Lipe^tm2Rze. (J:74644)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Lipe Mutation: 36 strains or lines available

References

• Original: J:74644 Haemmerle G, et al., Hormone-sensitive lipase deficiency in mice causes diglyceride accumulation in adipose tissue, muscle, and testis. J Biol Chem. 2002 Feb 15;277(7):4806-15
• All: 17 reference(s)