Mip^{Gt(OST1231)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Mip^{Gt(OST1231)Lex}
• Name: major intrinsic protein of lens fiber; gene trap OST1231, Lexicon Genetics
• MGI ID: MGI:1861978
• Synonyms: AQP0^-, Mip^{Gt(Betageo)513Lex}, Mip^{Gt(VICTR20)8Lex}, Mip^tm1Lex
• Gene: Mip Location: Chr10:128061707-128067681 bp, + strand Genetic Position: Chr10, 76.49 cM, cytoband D1
• Alliance: Mip^{Gt(OST1231)Lex} page

Mutation origin

• Mutant Cell Line: OST1231
• Germline Transmission: Earliest citation of germline transmission: J:84775
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene trap vector consisting of LTR sites flanking a beta-geo cassette containing a splice acceptor and a PGK-puro cassette containing a splice donor inserted into exon 1 of the endogenous gene, 168 nucleotides downstream of the start codon. The integration generated a nonsense mutation at codon 57, precluding the translation of ~79% (207 residues) of the protein, including the functionally conserved asparagine-proline-alanine motifs located at codons 68-70 and 184-186. Northern and Western blot analyses indicated an absence of normal transcript and protein in homozygous mutant mice. (J:84775)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST1231	CG472657 ; (MGI Seq Detail)	3' RACE	Chr10:128062916-128066374 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  24 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	18 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mip Mutation: 31 strains or lines available

References

• Original: J:84775 Shiels A, et al., Optical dysfunction of the crystalline lens in aquaporin-0-deficient mice. Physiol Genomics. 2001 Dec 21;7(2):179-86
• All: 14 reference(s)