Phenotypes associated with this allele

• Allele Symbol: Mip^{Gt(OST1231)Lex}
• Allele Name: gene trap OST1231, Lexicon Genetics
• Allele ID: MGI:1861978
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mip^Gt(OST1231)Lex/Mip^Gt(OST1231)Lex	involves: 129S5/SvEvBrd * C57BL/6J	MGI:2670087
ht2	Mip^Gt(OST1231)Lex/Mip^+	involves: 129S5/SvEvBrd * C57BL/6J	MGI:2670089

Genotype
MGI:2670087

hm1

• Allelic Composition: Mip^{Gt(OST1231)Lex}/Mip^{Gt(OST1231)Lex}
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

vision/eye

abnormal lens morphology ( J:84775 )

• homozygous mutant lenses are more fragile and prone to rupture upon dissection

• when lenses are adjusted for dry weight, the water content of homozygous mutant lenses is increased by 6% relative to wild-type lenses (~77.8% versus ~71.8%, respectively)

cataract ( J:84775 )

• at 3 weeks of age, homozygous mutant lenses viewed from the anterior or posterior poles under oblique illumination display dense polymorphic opacities surrounded by vacuolated and translucent regions of light scattering

• bilateral and fully penetrant lens opacities are first evident at ~P14 (upon eyelid opening)

• lens opacities are nearly complete (total opacities) at 5-6 months of age in the absence of microphthalmia

abnormal eye physiology ( J:84775 )

• at 3-4 weeks of age, homozygous mutant lenses exhibit an ~80% reduction in osmotic water permeability (P_f) values relative to wild-type lenses, measured in vitro as the shrinkage rate of fiber cell membrane vesicles in response to an osmotic challenge of 300-450 mM saline

Genotype
MGI:2670089

ht2

• Allelic Composition: Mip^{Gt(OST1231)Lex}/Mip⁺
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J

vision/eye

abnormal lens morphology ( J:84775 )

• when lenses are adjusted for dry weight, the water content of heterozygous mutant lenses is increased by 3.8% relative to wild-type lenses (~75.6% versus ~71.8%, respectively)

cataract ( J:84775 )

• heterozygotes display transparent lenses at 3 weeks of age, but develop bilateral and fully penetrant lens opacities by ~24 weeks of age

• at 3 weeks of age, heterozygous mutant lenses viewed from the anterior or posterior poles under oblique illumination show an abnormal transparent zone of light refraction; this optically distorted region is similar to patterned glass and extends from the central core or nucleus into the peripheral cortex of the lens

abnormal eye physiology ( J:84775 )

• at 3-4 weeks of age, heterozygous mutant lenses exhibit an ~54% reduction in osmotic water permeability (P_f) values relative to wild-type lenses, measured in vitro as the shrinkage rate of lens fiber cell membrane vesicles in response to an osmotic challenge of 300-450 mM saline

• at 5-6 months of age, heterozygous mutant lenses display a significantly impaired focusing power relative to wild-type lenses, as indicated by laser scan profiles