About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:348275 He XD, et al., TATA-binding associated factors have distinct roles during early mammalian development. Dev Biol. 2024 Jul;511:53-62
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7641384
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog (RCAB0002P-F/ RCAB001P)
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 2A - Control: embryonic day 3.5 (more )
Note: Triple labeled: magenta - Nanog; green - Sox17 (assay MGI:7641386); blue - DAPI.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 2A'' , 2A Expression is seen in the prospective epiblast.
Specimen 2B - Taf5-/-: embryonic day 3.5; Taf5em1(IMPC)J/Taf5em1(IMPC)J (more )
Note: Triple labeled: magenta - Nanog; green - Sox17 (assay MGI:7641386); blue - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Present 2B'' , 2B In the mutant the area of expression is no longer limited to the prospective epiblast.
Specimen 2C - Taf12-/-: embryonic day 3.5; Taf12em1(IMPC)Mbp/Taf12em1(IMPC)Mbp (more )
Note: Triple labeled: magenta - Nanog; green - Sox17 (assay MGI:7641386); blue - DAPI.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 2C'' , 2C As in wild type, expression is seen in the prospective epiblast.
Specimen 4A - Littermate: embryonic day 6.5 (more )
Note: Multi-labeled: magenta - Nanog; cyan - Cdh1 (assay MGI:7641392); yellow - Cdx2 (assay MGI:7641387); white - Pou5f1 (assay MGI:7641394); blue - DAPI.
Structure Level Pattern Image Note
TS8: epiblast Present 4A'''' , 4A
Specimen 4B - Taf13-/-: embryonic day 6.5; Taf13em1(IMPC)J/Taf13em1(IMPC)J (more )
Note: Multi-labeled: magenta - Nanog; cyan - Cdh1 (assay MGI:7641392); yellow - Cdx2 (assay MGI:7641387); white - Pou5f1 (assay MGI:7641394); blue - DAPI.
Structure Level Pattern Image Note
TS8: epiblast Absent 4B , 4B''''
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory