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Gene Expression Data
Assay Details
Assay
Reference: J:338194 Sulic AM, et al., Transcriptomic landscape of early hair follicle and epidermal development. Cell Rep. 2023 Jun 13;42(6):112643
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7574119
Gene symbol: Epcam
Gene name: epithelial cell adhesion molecule
Antibody: anti-CD326/Epcam (552370)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1F EpCAM top: embryonic day 15.5; Cyfip2tm1a(EUCOMM)Wtsi/Cyfip2+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Triple labeled: white - Epcam; magenta - Cyfip2 reporter (assay MGI:7574113); green - Shh reporter (assay MGI:7574114).
Structure Level Pattern Image Note
TS23: back skin Present 1F merged top , 1F Shh-GFP top , 1F Cyfip2-LacZ top
Specimen 1F EpCAM bottom: embryonic day 15.5; Cyfip2tm1a(EUCOMM)Wtsi/Cyfip2+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Triple labeled: white - Epcam; magenta - Cyfip2 reporter (assay MGI:7574113); green - Shh reporter (assay MGI:7574114).
Structure Level Pattern Image Note
TS23: hair epidermal placode Present 1F Cyfip2-LacZ bottom , 1F merged bottom , 1F Shh-GFP bottom
Specimen S1J EpCAM Control: embryonic day 15.5; Cyfip2tm1a(EUCOMM)Wtsi/Cyfip2+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: white - Epcam; green - Shh reporter (assay MGI:7574114).
Structure Level Pattern Image Note
TS23: hair epidermal placode Present S1J EpCAM Shh-GFP Control
Specimen S1J EpCAM LacZ/LacZ: embryonic day 15.5; Cyfip2tm1a(EUCOMM)Wtsi/Cyfip2tm1a(EUCOMM)Wtsi, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: white - Epcam; green - Shh reporter (assay MGI:7574114).
Structure Level Pattern Image Note
TS23: hair epidermal placode Present S1J EpCAM Shh-GFP LacZ/LacZ
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory