Gene Expression Data
Assay Details
Assay
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Reference:
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J:51656
Marcus RC, et al., Domains of regulatory gene expression and the developing optic chiasm: correspondence with retinal axon paths and candidate signaling cells. J Comp Neurol. 1999 Jan 18;403(3):346-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7539094
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe
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Probe preparation:
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Antisense RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 11.0-11.5
(more )
Note: Double labeled: brown - Shh; blue - Dlx2 (assay MGI:7539087).
Specimen
2A:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.0-11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: brown - Shh; blue - Dlx2 (assay MGI:7539087).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
2A
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Expression is in the preoptic area and the medial ganglionic eminence.
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TS19: diencephalon |
Present |
Regionally restricted |
2A
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Expression is in the mammillary, tuberal, and retrochiasmatic areas in the hypothalamus.
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Specimen
2B:
embryonic day 11.0-11.5
(more )
Note: Double labeled: brown - Shh; blue - Dlx2 (assay MGI:7539087).
Specimen
2B:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.0-11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
|
Double labeled: brown - Shh; blue - Dlx2 (assay MGI:7539087).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
2B
|
Expression is in the preoptic area and the medial ganglionic eminence.
|
TS19: diencephalon |
Present |
Regionally restricted |
2B
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Expression is in the mammillary, tuberal, and retrochiasmatic areas in the hypothalamus.
|
Specimen
2C:
embryonic day 11.0-11.5
(more )
Note: Double labeled: brown - Shh; blue - Dlx2 (assay MGI:7539087).
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.0-11.5
|
Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Agarose
|
Note:
|
Double labeled: brown - Shh; blue - Dlx2 (assay MGI:7539087).
|
Specimen
2D:
embryonic day 11.0-11.5
(more )
Note: Double labeled: brown - Shh; blue - Nkx2-2 (assay MGI:7539088).
Specimen
2D:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 11.0-11.5
|
Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: brown - Shh; blue - Nkx2-2 (assay MGI:7539088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
2D
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Expression is in the preoptic area and the medial ganglionic eminence.
|
TS19: diencephalon |
Present |
Regionally restricted |
2D
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Expression is in the mammillary, tuberal, and retrochiasmatic areas in the hypothalamus.
|
Specimen
2E:
embryonic day 11.0-11.5
(more )
Note: Double labeled: brown - Shh; blue - Nkx2-2 (assay MGI:7539088).
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6J
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Age:
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embryonic day 11.0-11.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: brown - Shh; blue - Nkx2-2 (assay MGI:7539088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
2E
|
Expression is in the preoptic area and the medial ganglionic eminence.
|
TS19: diencephalon |
Present |
Regionally restricted |
2E
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Expression is in the mammillary, tuberal, and retrochiasmatic areas in the hypothalamus.
|
Specimen
2F:
embryonic day 11.0-11.5
(more )
Note: Double labeled: brown - Shh; blue - Nkx2-2 (assay MGI:7539088).
Specimen
2F:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 11.0-11.5
|
Sex:
|
Not Specified
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Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Double labeled: brown - Shh; blue - Nkx2-2 (assay MGI:7539088).
|
|