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Gene Expression Data
Assay Details
Assay
Reference: J:183829 Correa SM, et al., Sex reversal in C57BL/6J XY mice caused by increased expression of ovarian genes and insufficient activation of the testis determining pathway. PLoS Genet. 2012 Apr;8(4):e1002569
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7524635
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: anti-Sox9
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3C/D Wt1+/+;Sf1+/+ XY B6: embryonic day 13.5 (more )
Note: Double labeled: green - Sox9; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 3C , 3D
Specimen 3E/F Wt1+/-;Sf1+/+ XY B6: embryonic day 13.5; Wt1tm1Jae/Wt1+ (more )
Note: Generated from B6 Wt1+/- and B6 +/+ XY B6 crosses. Double labeled: green - Sox9; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 3F , 3E Testis cords were less developed and more disorganized than cords in the wild-type. B6 Wt1+/-; Sf1+/+ gonads, however, were more similar to wild-type gonads than were Wt1+/+; Sf1+/- gonads.
Specimen 3G/H Wt1+/+;Sf1+/- XY B6: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+ (more )
Note: Double labeled: green - Sox9; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 3H , 3G Testis cords were less developed and more disorganized than cords in the wild-type.
Specimen 3I/J Wt1+/-;Sf1+/- XY B6: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+, Wt1tm1Jae/Wt1+ (more )
Note: Gonads differentiated as ovotestes. Double labeled: green - Sox9; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present Regionally restricted 3J , 3I Expressed in Sertoli cells in the anterior and posterior poles, but were not organized into cords.
Specimen not shown Wt1+/- XY AKR: embryonic day 13.5; Wt1tm1Jae/Wt1+ (more )
Note: Gonads that developed as ovaries.
Structure Level Pattern Image Note
TS21: gonad Absent Developed as ovaries, similar to XX ovaries.
Specimen not shown Sf1+/- XY AKR: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+ (more )
Note: Gonads that developed as ovaries.
Structure Level Pattern Image Note
TS21: gonad Absent Developed as ovaries, similar to XX ovaries.
Specimen not shown Wt1+/- XY AKR: embryonic day 13.5; Wt1tm1Jae/Wt1+ (more )
Note: Morphologically ovarian but expressed testicular markers (20%).
Structure Level Pattern Image Note
TS21: gonad Present Ovarian morphologically but contained positive cells.
Specimen not shown Sf1+/- XY AKR: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+ (more )
Note: Morphologically ovarian but expressed testicular markers (20%).
Structure Level Pattern Image Note
TS21: gonad Present Ovarian morphologically but contained positive cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory