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Gene Expression Data
Assay Details
Assay
Reference: J:330602 Zhou T, et al., Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice. Nat Commun. 2022 Oct 24;13(1):6233
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7451198
Gene symbol: Gfap
Gene name: glial fibrillary acidic protein
Antibody: anti-GFAP (clone G-A-5)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The authors reported using two Gfap antibodies but did not specify which was used to obtain these data; it is possible MGI:5615208 was used.
Results
Specimen S1b - cortex: postnatal month 2; Cx3cr1tm1Litt/Cx3cr1+ (more )
Note: Double labeled: red/magenta - Gfap; green - Cx3cr1 reporter (assay MGI:7451215).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex astrocyte Present S1b CD lower left , S1b CD cortex
Specimen S1b - hippocampus: postnatal month 2; Cx3cr1tm1Litt/Cx3cr1+ (more )
Note: Double labeled: red/magenta - Gfap; green - Cx3cr1 reporter (assay MGI:7451215).
Structure Cell Type Level Pattern Image Note
TS28: hippocampus astrocyte Present S1b CD lower center , S1b CD hippocampus
Specimen S1b - olfactory bulb: postnatal month 2; Cx3cr1tm1Litt/Cx3cr1+ (more )
Note: Double labeled: red/magenta - Gfap; green - Cx3cr1 reporter (assay MGI:7451215).
Structure Cell Type Level Pattern Image Note
TS28: main olfactory bulb astrocyte Present S1b CD lower right , S1b CD olfactory bulb
Specimen S9b - astrocyte: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: red - Gfap; green - P2ry12 reporter (assay MGI:7450957).
Structure Cell Type Level Pattern Image Note
TS28: brain astrocyte Present S9b astrocyte
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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