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Gene Expression Data
Assay Details
Assay
Reference: J:330602 Zhou T, et al., Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice. Nat Commun. 2022 Oct 24;13(1):6233
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7450957
Gene symbol: P2ry12
Gene name: purinergic receptor P2Y, G-protein coupled 12
Reporter: GFP
Antibody: anti-GFP (ab13970)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen S9b - cortex microglia: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Aif1 (assay MGI:7451199).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex microglial cell Present Widespread S9b cortex Expression is seen in almost all microglia.
Specimen S9b - meningeal: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Aif1 (assay MGI:7451199).
Structure Cell Type Level Pattern Image Note
TS28: brain meninges meningeal macrophage Absent S9b meningeal Expression is not present in non-myeloid cells in the brain.
Specimen S9b - choroid plexus: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Aif1 (assay MGI:7451199).
Structure Cell Type Level Pattern Image Note
TS28: choroid plexus choroid plexus macrophage Absent S9b choroid plexus Expression is not present in non-myeloid cells in the brain.
Specimen S9b - perivascular: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Adgre1 (assay MGI:7451191); blue - laminin.
Structure Cell Type Level Pattern Image Note
TS28: brain perivascular macrophage Absent S9b perivascular Expression is not present in non-myeloid cells in the brain.
Specimen S9b - neuron: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Rbfox3 (assay MGI:7451193).
Structure Cell Type Level Pattern Image Note
TS28: brain central nervous system neuron Absent S9b neuron Expression is not present in non-myeloid cells in the brain.
Specimen S9b - astrocyte: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Gfap (assay MGI:7451198).
Structure Cell Type Level Pattern Image Note
TS28: brain astrocyte Absent S9b astrocyte Expression is not present in non-myeloid cells in the brain.
Specimen S9b - pericyte: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Pdgfrb (assay MGI:7451196).
Structure Cell Type Level Pattern Image Note
TS28: brain brain pericyte Absent S9b pericyte Expression is not present in non-myeloid cells in the brain.
Specimen S9b - endothelial cell: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Pecam1 (assay MGI:7451197).
Structure Cell Type Level Pattern Image Note
TS28: brain vascular element brain microvascular endothelial cell Absent S9b endotherial Expression is not present in non-myeloid cells in the brain.
Specimen S9b - VSMC: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Acta2 (assay MGI:7451190).
Structure Cell Type Level Pattern Image Note
TS28: brain vascular element vascular associated smooth muscle cell Absent S9b VSMC Expression is not present in non-myeloid cells in the brain.
Specimen S9b - OPC: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Pdgfra (assay MGI:7451195).
Structure Cell Type Level Pattern Image Note
TS28: brain oligodendrocyte precursor cell Absent S9b OPC Expression is not present in non-myeloid cells in the brain.
Specimen S9b - OL: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Olig2 (assay MGI:7451194).
Structure Cell Type Level Pattern Image Note
TS28: brain oligodendrocyte Absent S9b OL Expression is not present in non-myeloid cells in the brain.
Specimen S9b - NSC: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: green - P2ry12 reporter; red - Nes (assay MGI:7451192).
Structure Cell Type Level Pattern Image Note
TS28: brain neural stem cell Absent S9b NSC Expression is not present in non-myeloid cells in the brain.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory