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Gene Expression Data
Assay Details
Assay
Reference: J:331142 Han P, et al., Cbln1 regulates axon growth and guidance in multiple neural regions. PLoS Biol. 2022 Nov;20(11):e3001853
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388461
Gene symbol: Isl2
Gene name: insulin related protein 2 (islet 2)
Antibody: anti-Isl1/2
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen S1E Isl1/2: embryonic day 11.0 (more )
Note: Triple labeled: blue - Isl1 and Isl2; green - Cbln1 (assay MGI:7388453); red - red - Pou4f1 (assay MGI:7388458).
Structure Cell Type Level Pattern Image Note
TS18: future spinal cord commissural neuron Present Regionally restricted S1E (merged) , S1E Isl1/2 Expression in dI3 dorsal commissural neurons.
Specimen S2E Cbln1fl/fl Isl1/2: embryonic day 11.5; Cbln1tm1.1Sjj/Cbln1tm1.1Sjj (more )
Note: Double labeled: green - Isl1 and Isl2; red - Pou4f1 (assay MGI:7388458).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord commissural neuron Present Regionally restricted S2E Cbln1fl/fl (merged) , S2E Cbln1fl/fl Isl1/2 Expression in dI3 dorsal commissural neurons.
Specimen S2E Wnt1-Cre Isl1/2: embryonic day 11.5; Cbln1tm1.1Sjj/Cbln1tm1.1Sjj, H2az2Tg(Wnt1-cre)11Rth/0 (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Pou4f1 (assay MGI:7388458).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord commissural neuron Present Regionally restricted S2E Wnt1-Cre Isl1/2 , S2E Wnt1-Cre (merged) Patterning not affected for dI3 neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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