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Gene Expression Data
Assay Details
Assay
Reference: J:308699 Bjorke B, et al., Oculomotor nerve guidance and terminal branching requires interactions with differentiating extraocular muscles. Dev Biol. 2021 Aug;476:272-281
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7378840
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4E: embryonic day 12.5; Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sor+ (more )
Note: Double labeled: red - Acta2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: extrinsic ocular pre-muscle mass Present 4E' , 4E Expressed in the superior, lateral and inferior rectus extraocular muscles, and a few muscle fibers of the superior oblique.
TS20: blood vessel Present 4E , 4E'
Specimen 4F: embryonic day 12.5; Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sor+, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: blood vessel Present 4F
TS20: extrinsic ocular pre-muscle mass Trace 4F Only expressed in a few scattered muscle fibers in 1 out of 5 mutant.
Specimen 4G: embryonic day 12.5; Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sor+, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: extrinsic ocular pre-muscle mass Trace 4G Only expressed in a few scattered muscle fibers in 1 out of 5 mutant.
TS20: blood vessel Present 4G
Specimen 4H: embryonic day 12.5; Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sor+, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: extrinsic ocular pre-muscle mass Trace 4H Only expressed in a few scattered muscle fibers in 1 out of 5 mutant.
TS20: blood vessel Present 4H
Specimen 5H: embryonic day 12.5 (more )
Note: Double labeled: red - Acta2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: blood vessel Present 5H
TS20: extrinsic ocular pre-muscle mass Present 5H
Specimen 5I: embryonic day 12.5; Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: red - Acta2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: blood vessel Present 5I No extrinsic ocular pre-muscle mass fibers were seen.
Specimen 5J: embryonic day 12.5; Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: red - Acta2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: blood vessel Present 5J No extrinsic ocular pre-muscle mass fibers were seen.
Specimen 5K: embryonic day 12.5; Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: red - Acta2; green - Tubb3 (assay MGI:7378506).
Structure Level Pattern Image Note
TS20: blood vessel Present 5K No extrinsic ocular pre-muscle mass fibers were seen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory