Antibody:	anti-Tuj1 (rabbit polyclonal 802001)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		2A	The oculomotor nerve maintained a plexus in the central location with Pitx2+ cells.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		2B	The oculomotor nerve maintained a plexus in the central location with Pitx2+ cells, which was devoid of myofibers as marked by myosin heavy chain antibody. The nerve did form small dorsal axon projections to initiate the superior branch and likely the branch to the inferior oblique.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		2C	Despite the initial formation of the superior and inferior branches, these nerves did not yet contact the nascent myofibers.

Structure	Level	Pattern	Image	Note
TS21: oculomotor nerve	Present		2D	Not penetrated into Pitx2+ muscle fibers around the eye, except the superior rectus by the superior branch.

Structure	Level	Pattern	Image	Note
TS21: oculomotor nerve	Present		2E	Not penetrated into Pitx2+ muscle fibers around the eye, except the superior rectus by the superior branch.

Structure	Level	Pattern	Image	Note
TS21: oculomotor nerve	Present		2F	Not penetrated into Pitx2+ muscle fibers around the eye, except the superior rectus by the superior branch.

Structure	Level	Pattern	Image	Note
TS21: oculomotor nerve	Present		2G	Nerves contacted and began to penetrate each muscle.

Structure	Level	Pattern	Image	Note
TS21: oculomotor nerve	Present		2H	Nerves contacted and began to penetrate each muscle.

Structure	Level	Pattern	Image	Note
TS21: oculomotor nerve	Present		2I	Nerves contacted and began to penetrate each muscle.

Structure	Level	Pattern	Image	Note
TS22: oculomotor nerve	Present		2L	Each muscle received innervation. The motor nerves formed an adult pattern.

Structure	Level	Pattern	Image	Note
TS17: oculomotor nerve	Present	Regionally restricted	4A	Expressed in the oculomotor nerve, which terminated when projecting into the mass of Pitx2+ cells ventrolateral to the eye.

Structure	Level	Pattern	Image	Note
TS17: oculomotor nerve	Present	Regionally restricted	4B	Expressed in the oculomotor nerve, which arrived in this region in a normal position.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		4C
TS20: optic nerve	Present		4C	Pitx2+ condensing muscles beginning to form in an array around the optic nerve. Additional Pitx2+ cells formed layers around the optic nerve and cup.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		4D
TS20: optic nerve	Present		4D	The Pitx2+ masses in the expected muscle positions were much smaller, and less densely packed, while Pitx2+ layers remained around the optic nerve and cup.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		4E' , 4E
TS20: optic nerve	Present		4E' , 4E
TS20: trigeminal ganglion	Present		4E' , 4E
TS20: trochlear nerve	Present	Regionally restricted	4E , 4E'	Expressed in the oculomotor IV nerve.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		4F , 4F'	Branches frequently merged into trigeminal branches.
TS20: trochlear nerve	Present	Regionally restricted	4F' , 4F	Expressed in the oculomotor IV/trochlear nerve. Branches frequently merged into trigeminal branches.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		4G' , 4G	Branches frequently merged into trigeminal branches.
TS20: trochlear nerve	Present	Regionally restricted	4G , 4G'	Expressed in the oculomotor IV/trochlear nerve. Branches frequently merged into trigeminal branches.

Structure	Level	Pattern	Image	Note
TS20: trochlear nerve	Present	Regionally restricted	4H' , 4H	Expressed in the oculomotor IV/trochlear nerve. Branches frequently merged into trigeminal branches.
TS20: oculomotor nerve	Present		4H , 4H'	Branches frequently merged into trigeminal branches.

Structure	Level	Pattern	Image	Note
TS17: oculomotor nerve	Present	Regionally restricted	5B	Expressed in the oculomotor nerve pioneers projecting out to the eye. The nerve ending was within a dense mass of cells, ventrolateral to the eye.

Structure	Level	Pattern	Image	Note
TS17: oculomotor nerve	Present	Regionally restricted	5C	Expressed in the oculomotor nerve projected to the ventrolateral area of the eye, but this area of mesenchyme failed to form the dense cell mass. Abnormal split in the nerve, instead of defasciculated axons in wild type.

Structure	Level	Pattern	Image	Note
TS17: oculomotor nerve	Present	Regionally restricted	5C'	Expressed in the oculomotor nerve fibers that projected past their normal stopping point, instead reaching close to the optic stalk.

Structure	Level	Pattern	Image	Note
TS19: oculomotor nerve	Present	Regionally restricted	5D	Expressed in a plexus of defasciculated oculomotor axons in a compact area.

Structure	Level	Pattern	Image	Note
TS19: oculomotor nerve	Present	Regionally restricted	5E	Expressed in widely projecting oculomotor axons, projecting abnormally both dorsal and ventral around the eye.

Structure	Level	Pattern	Image	Note
TS19: oculomotor nerve	Present	Regionally restricted	5F	Expressed in widely projecting oculomotor axons, projecting abnormally both dorsal and ventral around the eye.

Structure	Level	Pattern	Image	Note
TS19: oculomotor nerve	Present	Regionally restricted	5G	Expressed in widely projecting oculomotor axons, projecting abnormally both dorsal and ventral around the eye.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		5H , 5H'
TS20: trigeminal ganglion	Present		5H , 5H'
TS20: trochlear nerve	Present	Regionally restricted	5H , 5H'	Expressed in the oculomotor IV nerve. Trochlear axons were normally more defasciculated.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		5I' , 5I	Branches frequently merged into trigeminal branches. The oculomotor nerve often stopped prematurely, forming abnormal branches before the eye, while other branches projected around the dorsal and ventral eye.
TS20: trochlear nerve	Present	Regionally restricted	5I , 5I'	Expressed in the trochlear nerve projected abnormally close to the eye, or appeared to terminate prematurely. Trochlear axons did not form prominent ectopic branches, but many trochlear axons strayed to fasciculate with trigeminal or oculomotor nerve bundles.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present		5J , 5J'	Branches frequently merged into trigeminal branches. The oculomotor nerve often stopped prematurely, forming abnormal branches before the eye, while other branches projected around the dorsal and ventral eye.
TS20: trochlear nerve	Present	Regionally restricted	5J' , 5J	Expressed in the trochlear nerve projected abnormally close to the eye, or appeared to terminate prematurely. Trochlear axons did not form prominent ectopic branches, but many trochlear axons strayed to fasciculate with trigeminal or oculomotor nerve bundles.

Structure	Level	Pattern	Image	Note
TS20: trochlear nerve	Present	Regionally restricted	5K , 5K'	Expressed in the trochlear nerve projected abnormally close to the eye, or appeared to terminate prematurely. Trochlear axons did not form prominent ectopic branches, but many trochlear axons strayed to fasciculate with trigeminal or oculomotor nerve bundles.
TS20: oculomotor nerve	Present		5K , 5K'	Branches frequently merged into trigeminal branches. The oculomotor nerve often stopped prematurely, forming abnormal branches before the eye, while other branches projected around the dorsal and ventral eye.