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Gene Expression Data
Assay Details
Assay
Reference: J:105896 Ashique AM, et al., Localization of the scaffolding protein RACK1 in the developing and adult mouse brain. Brain Res. 2006 Jan 19;1069(1):31-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7378130
Gene symbol: Rack1
Gene name: receptor for activated C kinase 1
Antibody: anti-RACK1 (IgM)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 2A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon Present Graded 2F , 2A , 2D Expression is in a high-dorsal to low-ventral gradient.
TS19: telencephalon Present Graded 2F , 2A , 2D Expression is in a high-dorsal to low-ventral gradient.
Specimen 2B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: midbrain Present Graded 2B Expression is in a high-dorsal to low-ventral gradient.
Specimen 2C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: hindbrain Present Graded 2C Expression is in a high-dorsal to low-ventral gradient.
TS19: midbrain Present Graded 2C Expression is in a high-dorsal to low-ventral gradient.
Specimen 2J: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: telencephalon ventricular layer Strong 2J
TS21: cerebral cortex Strong Graded 2J Expression is in a high-rostral to low-caudal gradient.
TS21: choroid plexus Present 2J
TS21: lateral ganglionic eminence Present 2J
TS21: medial ganglionic eminence Present 2J
TS21: telencephalon Present Regionally restricted 2J Expression is in the hippocampus.
TS21: thalamus Weak 2J
Specimen 2K: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex marginal layer Strong 2K
TS21: cortical subplate Strong 2K
Specimen 2L: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex Strong Graded 2L Expression is in a high-rostral to low-caudal gradient which shifts rostrally in more lateral regions of the developing cortex.
TS21: choroid plexus Present 2L
TS21: lateral ganglionic eminence Present 2L
TS21: medial ganglionic eminence Present 2L
TS21: telencephalon Present Regionally restricted 2L Expression is in the hippocampus.
Specimen 4H: postnatal month 3 (more )
Note: Double labeled: red - Rack1; green - Glul (assay MGI:7378135).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex pyramidal neuron Strong 4J , 4H Pyramidal neurons are strongly positive and some glial cells are weakly positive.
TS28: cerebral cortex glial cell Weak 4J , 4H Pyramidal neurons are strongly positive and some glial cells are weakly positive.
Specimen 5A: postnatal month 3 (more )
Note: Double labeled: red - Rack1; green - Prkcb (assay MGI:7378136).
Structure Level Pattern Image Note
TS28: corpus striatum Strong 5B , 5A , 5D
Specimen 5E: postnatal month 3 (more )
Note: Double labeled: red - Rack1; green - Prkcb (assay MGI:7378136).
Structure Level Pattern Image Note
TS28: hippocampus CA1 Strong 5E , 5F , 5H
TS28: subiculum Strong 5E , 5F , 5H
Specimen 5I: postnatal month 3 (more )
Note: Double labeled: red - Rack1; green - Prkcb (assay MGI:7378136).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum granule cell layer neuron Present 5I , 5J , 5L Expression is in perikarya and proximal dendrites.
Specimen not shown: postnatal month 3 (more )
Note: Also stained for Rbfox3 (assay MGI:7378166).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex neuron Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory