Ncam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:240523 Yang Z, et al., Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016 Oct 01;418(1):66-74
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7346140
Gene symbol:	Ncam1
Gene name:	neural cell adhesion molecule 1

Antibody:	anti-NCAM [H28.123]
Detection system:	Secondary antibody

Results

1E left ©

Specimen 1E: postnatal day 0 (more

)
Note: Extended renal vesicle stage. Triple labeled: blue - Ncam1; red - Calb1 (assay MGI:7346142); green - Podxl2 (assay MGI:7346137).

Structure	Level	Pattern	Image	Note
TS27: renal vesicle	Present		1E left	Expressed in the plasma membrane.

1F ©

Specimen 1F: postnatal day 0 (more

)
Note: S-shaped body stage. Double labeled: blue - Ncam1; green - Podxl2 (assay MGI:7346137).

Structure	Level	Pattern	Image	Note
TS27: s-shaped body	Present		1F	Expressed in the plasma membrane.

2A left ©

2A right lower ©

Specimen 2A: postnatal day 0 (more

)
Note: Triple labeled: blue - Ncam1; red - Podxl2 (assay MGI:7346135); green - Rab11a (assay MGI:7346151) and Rab11b (assay MGI:7346428).

Structure	Level	Pattern	Image	Note
TS27: cap mesenchyme	Present		2A left , 2A right lower	Expressed in the plasma membrane.

2B left ©

Specimen 2B: postnatal day 0 (more

)
Note: Triple labeled: blue - Ncam1; red - Podxl2 (assay MGI:7346135); green - Rab11a (assay MGI:7346151) and Rab11b (assay MGI:7346428).

Structure	Level	Pattern	Image	Note
TS27: renal vesicle	Present		2B left	Expressed in the plasma membrane.

3A left ©

Specimen 3A: postnatal day 0 (more

)
Note: Triple labeled: blue - Ncam1; red - Podxl2 (assay MGI:7346135); green - Ezr (assay MGI:7346165).

Structure	Level	Pattern	Image	Note
TS27: renal vesicle	Present		3A left	Expressed in the plasma membrane.

3B left ©

Specimen 3B: postnatal day 0 (more

)
Note: Triple labeled: blue - Ncam1; red - Podxl2 (assay MGI:7346135); green - Ezr (assay MGI:7346165).

Structure	Level	Pattern	Image	Note
TS27: renal vesicle	Present		3B left	Expressed in the plasma membrane.

4A CTL left ©

4A CTL right lower ©

Specimen 4A CTL: postnatal day 0 (more

)
Note: Triple labeled: blue - Ncam1; green - Podxl2 (assay MGI:7346137); red - Prkci (assay MGI:7346175).

Structure	Level	Pattern	Image	Note
TS27: renal vesicle	Present		4A CTL right lower , 4A CTL left	Expressed in the plasma membrane.

4A tg/tg left ©

4A tg/tg right lower ©

Specimen 4A tg/tg: postnatal day 0; Podxl2^{tm1a(EUCOMM)Wtsi}/Podxl2^{tm1a(EUCOMM)Wtsi} (more

)
Note: Triple labeled: blue - Ncam1; green - Podxl2 (assay MGI:7346137); red - Prkci (assay MGI:7346175).

Structure	Level	Pattern	Image	Note
TS27: renal vesicle	Present		4A tg/tg right lower , 4A tg/tg left	Expressed in the plasma membrane.

5A CTL left ©

Specimen 5A CTL: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi} (more

)
Note: Triple labeled: blue - Ncam1; green - Pard6b (assay MGI:7346199); red - Podxl2 (assay MGI:7346135).

Structure	Level	Pattern	Image	Note
TS22: renal vesicle	Present		5A CTL right lower , 5A CTL left	Expressed in the plasma membrane.

5A CKO left ©

Specimen 5A cKO: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi}, Tg(Pax3-cre)1Joe/0 (more

)
Note: Conditional mutant. Triple labeled: blue - Ncam1; green - Pard6b (assay MGI:7346199); red - Podxl2 (assay MGI:7346135).

Structure	Level	Pattern	Image	Note
TS22: renal vesicle	Present		5A CKO right lower , 5A CKO left	Expressed in the plasma membrane, similar as in the control.

Specimen 6A CTL: postnatal day 0; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi} (more

)
Note: Double labeled: blue - Ncam1; green - Nherf1 (assay MGI:7346207).

Structure	Level	Pattern	Image	Note
TS27: s-shaped body	Present		6A right upper	Expressed in the plasma membrane.

Specimen 6A cKO: postnatal day 0; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi}, Tg(Pax3-cre)1Joe/0 (more

)
Note: Conditional mutant. Double labeled: blue - Ncam1; green - Nherf1 (assay MGI:7346207).

Structure	Level	Pattern	Image	Note
TS27: s-shaped body	Present		6A right lower	Expressed in the plasma membrane, similar as in the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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