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Gene Expression Data
Assay Details
Assay
Reference: J:240523 Yang Z, et al., Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016 Oct 01;418(1):66-74
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7346137
Gene symbol: Podxl2
Gene name: podocalyxin-like 2
Antibody: Mouse Endoglycan/PODXL2 Antibody (AF3534)
Detection system: Secondary antibody
Results
Specimen 1E: postnatal day 0 (more )
Note: Extended renal vesicle stage. Triple labeled: green - Podxl2; blue - Ncam1 (assay MGI:7346140); red - Calb1 (assay MGI:7346142).
Structure Level Pattern Image Note
TS27: renal vesicle Present Regionally restricted 1E right lower , 1E right upper , 1E left Expressed at the leading edge of an extending lumen and at the discrete de novo lumens. Also expressed in intracellular puncta of adjacent cells that had not yet polarized, and appeared to partially fill the lumen with large, circular structures (extracellular vesicles).
Specimen 1F: postnatal day 0 (more )
Note: S-shaped body stage. Double labeled: green - Podxl2; blue - Ncam1 (assay MGI:7346140).
Structure Cell Type Level Pattern Image Note
TS27: s-shaped body podocyte Present Regionally restricted 1F Expressed in the apical/luminal surface, including the proximal portion containing podocyte precursors.
Specimen 1G: postnatal day 0 (more )
Note: Pretubular aggregate stage. Triple labeled: green - Podxl2; blue - Prkci (assay MGI:7346141); red - Podxl (assay MGI:7346144).
Structure Level Pattern Image Note
TS27: pretubular aggregate Present 1G right upper , 1G left Expressed in the puncta, which appeared to cluster and form higher-order structures.
Specimen 1H: postnatal day 0 (more )
Note: Renal vesicle stage. Triple labeled: green - Podxl2; blue - Prkci (assay MGI:7346141); red - Podxl (assay MGI:7346144).
Structure Level Pattern Image Note
TS27: renal vesicle Present Regionally restricted 1H left , 1H right upper During lumen formation, positive puncta relocalized to the apical cell surface.
Specimen 1I: postnatal day 0 (more )
Note: S-shaped body stage. Triple labeled: green - Podxl2; blue - Prkci (assay MGI:7346141); red - Podxl (assay MGI:7346144).
Structure Cell Type Level Pattern Image Note
TS27: s-shaped body podocyte Present 1I left Expressed in the apical/luminal surface, including the proximal portion containing podocyte precursors.
Specimen 4A CTL: postnatal day 0 (more )
Note: Triple labeled: green - Podxl2; blue - Ncam1 (assay MGI:7346140); red - Prkci (assay MGI:7346175).
Structure Level Pattern Image Note
TS27: renal vesicle Present Regionally restricted 4A CTL right upper , 4A CTL left Positive puncta relocalized to the apical cell surface.
Specimen 4A tg/tg: postnatal day 0; Podxl2tm1a(EUCOMM)Wtsi/Podxl2tm1a(EUCOMM)Wtsi (more )
Note: Triple labeled: green - Podxl2; blue - Ncam1 (assay MGI:7346140); red - Prkci (assay MGI:7346175).
Structure Level Pattern Image Note
TS27: renal vesicle Absent 4A tg/tg left , 4A tg/tg right upper
Specimen S1: embryonic day 10.5 (more )
Note: Triple labeled: green - Podxl2; white - Cdh1 (assay MGI:7346248); red - Prkci (assay MGI:7346175).
Structure Level Pattern Image Note
TS17: pancreas primordium Present Regionally restricted S1_right inset upper , S1_left , S1_right , S1_right inset lower Expressed in many, but not all microlumens.
Specimen not shown: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: capillary loop renal corpuscle Weak
TS27: glomerular tuft Absent
TS27: renal tubule Absent
Specimen not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: glomerular tuft Absent
TS28: renal tubule Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory