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Gene Expression Data
Assay Details
Assay
Reference: J:304360 Dominguez-Bautista JA, et al., Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord. Front Cell Dev Biol. 2021;9:587096
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6886033
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: ISLET-1 antibody [EP4182] (ab109517)
Detection system: Secondary antibody
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Clone 40.2D6 MGI:1314839 may have been used in this assay.
Results
Specimen 1C: embryonic day 11.5 (more )
Note: Double labeled: red - Isl1; green - SA-Beta-gal (to reveal senescent cells).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord interneuron Present 1C ISLET-1 , 1C ISLET-1/SA-Beta-gal
TS19: future spinal cord motor neuron Present 1C ISLET-1 , 1C ISLET-1/SA-Beta-gal
Specimen 1D Medulla oblongata: embryonic day 13.5 (more )
Note: Double labeled: red - Isl1; green - Adgre1 (assay MGI:6886034). Also stained for endogenous SA-Beta-gal (to reveal senescent cells).
Structure Cell Type Level Pattern Image Note
TS21: medulla oblongata motor neuron Present 1D Medulla oblongata Merge , 1D Medulla oblongata ISLET-1
Specimen 1D Spinal cord: embryonic day 13.5 (more )
Note: Double labeled: red - Isl1; green - Adgre1 (assay MGI:6886034). Also stained for endogenous SA-Beta-gal (to reveal senescent cells).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord motor neuron Present 1D Spinal cord ISLET-1 , 1D Spinal cord Merge
Specimen 2C: embryonic day 10.5 (more )
Note: Multi-labeled: green - Isl1; red - Cdkn1a (assay MGI:6886035); green - Pecam1 (assay MGI:6886038); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord motor neuron Present 2C Merge + ISLET-1
Specimen 3A E10.5: embryonic day 10.5 (more )
Note: Double labeled: red - Isl1; green - Cdkn2a (assay MGI:6886042).
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord motor neuron Present 3A E10.5 Merge , 3A E10.5 ISLET-1
Specimen 3A E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Isl1; green - Cdkn2a (assay MGI:6886042).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord motor neuron Trace 3A E12.5 Merge , 3A E12.5 ISLET-1 Number of expressing cells decreases from E10.5 to E12.5.
Specimen 4 E10.5: embryonic day 10.5 (more )
Note: Double labeled: green - Isl1; red - Hes1 (assay MGI:6886044).
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord interneuron Present 4_E10.5 ISLET-1 , 4_E10.5 ISLET-1/HES1
TS17: future spinal cord motor neuron Present 4_E10.5 ISLET-1 , 4_E10.5 ISLET-1/HES1
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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