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Gene Expression Data
Assay Details
Assay
Reference: J:304360 Dominguez-Bautista JA, et al., Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord. Front Cell Dev Biol. 2021;9:587096
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6886035
Gene symbol: Cdkn1a
Gene name: cyclin dependent kinase inhibitor 1A
Antibody: anti-p21 [EPR3993] (ab109199)
Detection system: Secondary antibody
Results
Specimen 2A E10.5: embryonic day 10.5 (more )
Note: Double labeled: red - Cdkn1a; green - Pecam1 (assay MGI:6886038).
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord endothelial cell Present 2A E10.5 p21/PECAM , 2A E10.5 p21
Specimen 2A E11.5: embryonic day 11.5 (more )
Note: Double labeled: red - Cdkn1a; green - Pecam1 (assay MGI:6886038).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord endothelial cell Present 2A E11.5 p21/PECAM , 2A E11.5 p21
Specimen 2B E10.5: embryonic day 10.5 (more )
Note: Double labeled: red - Cdkn1a; blue - DAPI.
Structure Level Pattern Image Note
TS17: neural tube floor plate Absent 2B E10.5 p21 , 2B E10.5 p21/DAPI
TS17: notochord Present 2B E10.5 p21/DAPI , 2B E10.5 p21
Specimen 2B E11.5: embryonic day 11.5 (more )
Note: Double labeled: red - Cdkn1a; blue - DAPI.
Structure Level Pattern Image Note
TS19: notochord Present 2B E11.5 p21 , 2B E11.5 p21/DAPI
Specimen 2C: embryonic day 10.5 (more )
Note: Multi-labeled: red - Cdkn1a; green - Pecam1 (assay MGI:6886038); green - Isl1 (assay MGI:6886033); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord vascular element endothelial cell Present Regionally restricted 2C p21/DAPI , 2C Merge + ISLET-1 , 2C p21 , 2C p21/PECAM Expression in endothelial cells surrounding motoneuron column.
TS17: neural tube lateral wall motor neuron Absent 2C p21/DAPI , 2C Merge + ISLET-1 , 2C p21/PECAM , 2C p21
Specimen S1A: embryonic day 12.5 (more )
Note: Double labeled: green - Cdkn1a; blue - DAPI.
Structure Level Pattern Image Note
TS20: hand epithelium Present Regionally restricted S1A Merge , S1A p21 Expression in apical ectodermal ridge.
Specimen S1B upper: embryonic day 12.0 (more )
Note: Triple labeled: red - Cdkn1a; green - Pecam1 (assay MGI:6886038); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS20: medulla oblongata endothelial cell Present S1B p21/DAPI (upper) , S1B PECAM/p21 (upper) , S1B p21 (upper)
Specimen S1B lower: embryonic day 12.0 (more )
Note: Triple labeled: red - Cdkn1a; green - Pecam1 (assay MGI:6886038); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS20: medulla oblongata endothelial cell Present S1B PECAM/p21 (lower) , S1B p21/DAPI (lower) , S1B p21 (lower)
Specimen S1C: embryonic day 13.5 (more )
Note: Double labeled: red - Cdkn1a; green - Pecam1 (assay MGI:6886038).
Structure Cell Type Level Pattern Image Note
TS21: forelimb endothelial cell Present S1C PECAM/p21 , S1C p21
TS21: thoracic segment of trunk endothelial cell Present S1C PECAM/p21 , S1C p21
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory