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Gene Expression Data
Assay Details
Assay
Reference: J:314670 Thambyrajah R, et al., GFI1 proteins orchestrate the emergence of haematopoietic stem cells through recruitment of LSD1. Nat Cell Biol. 2016 Jan;18(1):21-32
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6828606
Gene symbol: Gfi1
Gene name: growth factor independent 1 transcription repressor
Reporter: GFP
Antibody: anti-GFP (NB100-1614)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4Ai: embryonic day 10.5; Gfi1tm2Tmo/Gfi1+, Gfi1btm1Tmo/Gfi1b+ (more )
Note: Multi-labeled: green - reporters for Gfi1 and Gfi1b (assay MGI:6828607); red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 4Ai GFP , 4Ai Merged Expression is seen in cells embedded within the endothelium and/or at the base of the intra-aortic haematopoietic clusters.
TS17: hematopoietic system Present Regionally restricted 4Ai GFP , 4Ai Merged Expression is seen in cells embedded within the endothelium and/or at the base of the intra-aortic haematopoietic clusters.
Specimen 4Aii: embryonic day 10.5; Gfi1tm2Tmo/Gfi1tm2Tmo, Gfi1btm1Tmo/Gfi1b+ (more )
Note: Multi-labeled: green - reporters for Gfi1 and Gfi1b (assay MGI:6828607); red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 4Aii GFP , 4Aii Merged Expression is seen in cells embedded within the endothelium and/or at the base of the intra-aortic haematopoietic clusters.
TS17: hematopoietic system Present Regionally restricted 4Aii GFP , 4Aii Merged Expression is seen in cells embedded within the endothelium and/or at the base of the intra-aortic haematopoietic clusters.
Specimen 4Aiii: embryonic day 10.5; Gfi1tm2Tmo/Gfi1+, Gfi1btm1Tmo/Gfi1btm1Tmo (more )
Note: Multi-labeled: green - reporters for Gfi1 and Gfi1b (assay MGI:6828607); red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 4Aiii GFP , 4Aiii Merged Expression is seen in cells embedded within the endothelium and/or at the base of the intra-aortic haematopoietic clusters.
TS17: hematopoietic system Present Regionally restricted 4Aiii GFP , 4Aiii Merged Expression is seen in cells embedded within the endothelium and/or at the base of the intra-aortic haematopoietic clusters.
Specimen 4Aiv: embryonic day 10.5; Gfi1tm2Tmo/Gfi1tm2Tmo, Gfi1btm1Tmo/Gfi1btm1Tmo (more )
Note: Multi-labeled: green - reporters for Gfi1 and Gfi1b (assay MGI:6828607); red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 4Aiv Merged , 4Aiv GFP Expression is seen in cells embedded within the endothelium.
TS17: hematopoietic system Absent 4Aiv Merged , 4Aiv GFP There are no expressing intra-aortic haematopoietic clusters.
Specimen S5Ai: embryonic day 10.5; Gfi1tm2Tmo/Gfi1tm2Tmo (more )
Note: Triple labeled: green - Gfi1 reporter; red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted S5Ai Merge , S5Ai GFI1 Expression is seen in cells embedded within the endothelium and/or at the base of the intra-aortic haematopoietic clusters.
TS17: hematopoietic system Present Regionally restricted S5Ai Merge , S5Ai GFI1 Expression is seen in cells embedded within the endothelium and/or at the base of the intra-aortic haematopoietic clusters.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory