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Gene Expression Data
Assay Details
Assay
Reference: J:314670 Thambyrajah R, et al., GFI1 proteins orchestrate the emergence of haematopoietic stem cells through recruitment of LSD1. Nat Cell Biol. 2016 Jan;18(1):21-32
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6827463
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4Ai: embryonic day 10.5; Gfi1tm2Tmo/Gfi1+, Gfi1btm1Tmo/Gfi1b+ (more )
Note: Multi-labeled: red - Pecam1; green - reporters for Gfi1 (assay MGI:6828606) and Gfi1b (assay MGI:6828607); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 4Ai CD31 , 4Ai Merged Expression is seen in the endothelium.
Specimen 4Aii: embryonic day 10.5; Gfi1tm2Tmo/Gfi1tm2Tmo, Gfi1btm1Tmo/Gfi1b+ (more )
Note: Multi-labeled: red - Pecam1; green - reporters for Gfi1 (assay MGI:6828606) and Gfi1b (assay MGI:6828607); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 4Aii Merged , 4Aii CD31 Expression is seen in the endothelium.
Specimen 4Aiii: embryonic day 10.5; Gfi1tm2Tmo/Gfi1+, Gfi1btm1Tmo/Gfi1btm1Tmo (more )
Note: Multi-labeled: red - Pecam1; green - reporters for Gfi1 (assay MGI:6828606) and Gfi1b (assay MGI:6828607); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 4Aiii CD31 , 4Aiii Merged Expression is seen in the endothelium.
Specimen 4Aiv: embryonic day 10.5; Gfi1tm2Tmo/Gfi1tm2Tmo, Gfi1btm1Tmo/Gfi1btm1Tmo (more )
Note: Multi-labeled: red - Pecam1; green - reporters for Gfi1 (assay MGI:6828606) and Gfi1b (assay MGI:6828607); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 4Aiv CD31 , 4Aiv Merged Expression is seen in the endothelium.
Specimen S5Ai: embryonic day 10.5; Gfi1tm2Tmo/Gfi1tm2Tmo (more )
Note: Triple labeled: red - Pecam1; green - Gfi1 reporter (assay MGI:6828606); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted S5Ai CD31 , S5Ai Merge Expression is seen in the endothelium.
Specimen S5Aii: embryonic day 10.5; Gfi1btm1Tmo/Gfi1btm1Tmo (more )
Note: Triple labeled: red - Pecam1; green - Gfi1b reporter (assay MGI:6828607); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted S5Aii CD31 , S5Aii Merge Expression is seen in the endothelium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory