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Gene Expression Data
Assay Details
Assay
Reference: J:314670 Thambyrajah R, et al., GFI1 proteins orchestrate the emergence of haematopoietic stem cells through recruitment of LSD1. Nat Cell Biol. 2016 Jan;18(1):21-32
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6828601
Gene symbol: Kit
Gene name: KIT proto-oncogene receptor tyrosine kinase
Antibody: anti-CD117 (2B8)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4Ai: embryonic day 10.5; Gfi1tm2Tmo/Gfi1+, Gfi1btm1Tmo/Gfi1b+ (more )
Note: Multi-labeled: yellow - Kit; red - Pecam1 (assay MGI:6827463); green - reporters for Gfi1 (assay MGI:6828606) and Gfi1b (assay MGI:6828607). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Clusters 4Ai c-KIT , 4Ai Merged Expression is seen in intra-aortic haematopoietic clusters.
Specimen 4Aii: embryonic day 10.5; Gfi1tm2Tmo/Gfi1tm2Tmo, Gfi1btm1Tmo/Gfi1b+ (more )
Note: Multi-labeled: yellow - Kit; red - Pecam1 (assay MGI:6827463); green - reporters for Gfi1 (assay MGI:6828606) and Gfi1b (assay MGI:6828607). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted 4Aii Merged , 4Aii c-KIT Expression is seen in intra-aortic haematopoietic clusters.
Specimen 4Aiii: embryonic day 10.5; Gfi1tm2Tmo/Gfi1+, Gfi1btm1Tmo/Gfi1btm1Tmo (more )
Note: Multi-labeled: yellow - Kit; red - Pecam1 (assay MGI:6827463); green - reporters for Gfi1 (assay MGI:6828606) and Gfi1b (assay MGI:6828607). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted 4Aiii c-KIT , 4Aiii Merged Expression is seen in intra-aortic haematopoietic clusters.
Specimen 4Aiv: embryonic day 10.5; Gfi1tm2Tmo/Gfi1tm2Tmo, Gfi1btm1Tmo/Gfi1btm1Tmo (more )
Note: Multi-labeled: yellow - Kit; red - Pecam1 (assay MGI:6827463); green - reporters for Gfi1 (assay MGI:6828606) and Gfi1b (assay MGI:6828607). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: hematopoietic system Absent 4Aiv Merged , 4Aiv c-KIT No expressing intra-aortic haematopoietic clusters are seen.
Specimen S5Ai: embryonic day 10.5; Gfi1tm2Tmo/Gfi1tm2Tmo (more )
Note: Triple labeled: yellow - Kit; red - Pecam1 (assay MGI:6827463); green - Gfi1 reporter (assay MGI:6828606). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Clusters S5Ai Merge , S5Ai c-KIT Expression is seen in intra-aortic haematopoietic clusters.
Specimen S5Aii: embryonic day 10.5; Gfi1btm1Tmo/Gfi1btm1Tmo (more )
Note: Triple labeled: yellow - Kit; red - Pecam1 (assay MGI:6827463); green - Gfi1b reporter (assay MGI:6828607). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Clusters S5Aii Merge , S5Aii c-KIT Expression is seen in intra-aortic haematopoietic clusters.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory