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Gene Expression Data
Assay Details
Assay
Reference: J:231833 Hashimoto R, et al., Origins of oligodendrocytes in the cerebellum, whose development is controlled by the transcription factor, Sox9. Mech Dev. 2016 May;140:25-40
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6444828
Gene symbol: Pdgfra
Gene name: platelet derived growth factor receptor, alpha polypeptide
Antibody: anti-CD140a
Results
Specimen 1B/C: embryonic day 18.5; En1tm2(cre)Wrst/En1+, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Cre reporter.
Structure Level Pattern Image Note
TS26: cerebellum Present Regionally restricted 1B , 1C Expression in oligodendrocyte progenitor cells (OPCs). All co-express En1-Cre reporter.
Specimen 1E/F: embryonic day 18.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Cre reporter.
Structure Level Pattern Image Note
TS26: cerebellum Present Regionally restricted 1E , 1F Expression in oligodendrocyte progenitor cells (OPCs). About 6% co-express Ptf1a-Cre reporter.
Specimen 1H/I: embryonic day 18.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Atoh1-cre)1Mhos/0 (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Cre reporter.
Structure Level Pattern Image Note
TS26: cerebellum Present Regionally restricted 1I , 1H Expression in oligodendrocyte progenitor cells (OPCs). No co-expression with Atoh1-Cre reporter.
Specimen 2G/H: embryonic day 11.5 (more )
Note: Double labeled: red - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS19: metencephalon basal plate Present Regionally restricted 2H , 2G Expression in oligodendrocyte progenitor cells (OPC) in ventral rhombomere 1 (r1).
Specimen 2I: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebellum Absent 2I Authors report little immunoreactivity at E14.5
Specimen 2J: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present Regionally restricted 2J Expression in cerebellar oligodendrocytes migrating from ventral rhombomere 1 (r1).
TS24: cerebellum Present 2J Expression in cerebellum beginning at E16.5.
Specimen 2K: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present Regionally restricted 2K Expression in cerebellar oligodendrocytes migrating from ventral rhombomere 1 (r1).
TS26: cerebellum Present Widespread 2K Increased expression at E18.5, spreading through whole cerebellar region.
Specimen 3H: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: metencephalon basal plate Present Regionally restricted 3H Expression in oligodendrocyte progenitor cells in mantle zone in vicinity of Olig2-expressing neuroepithelial (NE) domain.
Specimen 3L: embryonic day 12.5 (more )
Note: Double labeled: green - Pdgfra; pink - Sox9 (assay MGI:6444832).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Present Regionally restricted 3L Expression in oligodendrocyte progenitor cells (OPCs) in ventral rhombomere 1 (r1).
Specimen 3M: embryonic day 14.5 (more )
Note: Double labeled: green - Pdgfra; pink - Sox9 (assay MGI:6444832).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Present Regionally restricted 3M Expression in oligodendrocyte progenitor cells (OPCs) in ventral rhombomere 1 (r1).
Specimen 3P: embryonic day 16.5 (more )
Note: Double labeled: green - Pdgfra; pink - Sox9 (assay MGI:6444832).
Structure Level Pattern Image Note
TS24: cerebellum Present Regionally restricted 3P Expression in oligodendrocyte progenitor cells (OPCs) in cerebellum
Specimen 3Q: embryonic day 18.5 (more )
Note: Double labeled: green - Pdgfra; pink - Sox9 (assay MGI:6444832).
Structure Level Pattern Image Note
TS26: cerebellum Present Regionally restricted 3Q Expression in oligodendrocyte progenitor cells (OPCs) in cerebellum
Specimen E14.5 control not shown: embryonic day 14.5; En1tm2(cre)Wrst/En1+ (more )
Structure Level Pattern Image Note
TS22: cerebellum Absent
Specimen E14.5 mutant not shown: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: cerebellum Absent
Specimen 5A: embryonic day 16.5; En1tm2(cre)Wrst/En1+ (more )
Structure Level Pattern Image Note
TS24: cerebellum Ambiguous 5A
Specimen 5B: embryonic day 16.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cerebellum Present 5B In heterozygote, the number of expressing cells appeared to increase at E16.5
Specimen 5C: embryonic day 16.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cerebellum Weak 5C Fewer expressing cells in cerebellum at E16.5.
Specimen 5D: embryonic day 18.5; En1tm2(cre)Wrst/En1+ (more )
Structure Level Pattern Image Note
TS26: cerebellum Present 5D
Specimen 5E: embryonic day 18.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: cerebellum Trace 5E In heterozygote, the number of expressing cells appeared to decrease at E18.5
Specimen 5F: embryonic day 18.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: cerebellum Trace 5F Authors report even less expressing cells E16.5 compared to wild type.
Specimen 6A: embryonic day 11.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS19: metencephalon basal plate Ambiguous 6A
Specimen 6B: embryonic day 11.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS19: metencephalon basal plate Ambiguous 6B
Specimen 6C: embryonic day 11.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS19: metencephalon basal plate Ambiguous 6C
Specimen 6D: embryonic day 12.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Present Regionally restricted 6D Expression in some cells in mantle zone adjacent to Olig2-expression neuroepithelial (NE) region.
Specimen 6E: embryonic day 12.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Strong Regionally restricted 6E
Specimen 6F: embryonic day 12.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Absent 6F Expression not detectable in mantle zone at E12.5.
Specimen 6G: embryonic day 14.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Present Regionally restricted 6G Expression in mantle zone adjacent to Olig2-expression neuroepithelial (NE) region gradually increased as development proceeded.
Specimen 6H: embryonic day 14.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Strong Regionally restricted 6H Number of expressing cells higher than in control.
Specimen 6I: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Weak Regionally restricted 6I Expression appears at E14.5 in much fewer number compared to control.
Specimen 6J: embryonic day 16.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present Regionally restricted 6J Expression in mantle zone adjacent to Olig2-expression neuroepithelial (NE) region plateaued at E16.5 in control mice.
Specimen 6K: embryonic day 16.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present 6K
Specimen 6L: embryonic day 16.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present 6L
Specimen 6M: embryonic day 18.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 6M
Specimen 6N: embryonic day 18.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 6N
Specimen 6O: embryonic day 18.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: pink - Pdgfra; green - Olig2 (assay MGI:6444829).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 6O
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05/07/2024
MGI 6.23 		The Jackson Laboratory