Reporter:	GFP
Antibody:	anti-GFP (Nacalai)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS14: otic placode epithelium	Present	Widespread	3A/B
TS14: branchial arch	Present		3A/B

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Widespread	3C/D

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Widespread	3E/F
TS17: head mesenchyme	Present	Single cells	3E/F	Scattered expressing cells were detected in the medial and lateral sides of the vesicle, as well as in the dorsal part adjacent to the neural tube. It is thought that these are neural crest cells.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Present		3H , 3G	Expression is seen in the entire epithelium of the cochlear duct, vestibule and semicircular ducts.
TS19: semicircular canal epithelium	Present		3G	Expression is seen in the entire epithelium of the cochlear duct, vestibule and semicircular ducts.
TS19: vestibular component epithelium	Present		3G	Expression is seen in the entire epithelium of the cochlear duct, vestibule and semicircular ducts.
TS19: head mesenchyme	Present	Single cells	3G , 3H	Expressing cells were detected approaching the inner ear only on the lateral, but not the medial, side.

Structure	Level	Pattern	Image	Note
TS20: cochlea	Present		3I , 3J
TS20: inner ear	Present	Single cells	3I , 3J	Scattering expressing cells seem to approach the ventral side of the cochlear duct, a region that forms part of the stria vascularis later in development.

Structure	Level	Pattern	Image	Note
TS22: cochlea	Present		3L , 3K
TS22: inner ear	Present	Single cells	3K , 3L	Scattered expressing cells seem to approach the ventral side of the cochlea, where the developing stria vascularis is located.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present		3M , 3O/P , 3N	Expression is seen in the cochlear duct, including in pigmented cells (presumed melanocytes) in the intermediate cell layer of the stria vascularis.
TS24: cochlear duct epithelium	Present	Widespread	3O/P , 3M , 3N

Structure	Level	Pattern	Image	Note
TS27: cochlear duct	Present		4A	Expression is seen in cochlear duct epithelium but is not expressed in mesenchymal cells of the inner ear.
TS27: inner ear	Present	Regionally restricted	4A	Expression is seen in cochlear duct epithelium but is not expressed in mesenchymal cells of the inner ear.

Structure	Level	Pattern	Image	Note
TS27: osseus labyrinth vestibule	Present		4B	Expression is seen in the vestible.

Structure	Level	Pattern	Image	Note
TS27: cochlear duct	Present		4D , 4C	Expression is seen in the greater and lesser epithelial ridge, as well as in supporting cells; it is not seen in the hair cells.
TS27: stria vascularis	Present	Regionally restricted	4D , 4C	Expression is seen in the marginal cells.

Structure	Level	Pattern	Image	Note
TS27: vestibular epithelium	Present	Regionally restricted	4F , 4E	In vestibular epithelial regions, expression is seen in the supporting cells; it is not seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present	Regionally restricted	4I , 4H , 4G	Based on double staining experiments, the expressing cells were identified as glial cells.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present	Regionally restricted	4K , 4J , 4L	Based on double staining experiments, the expressing cells were identified as glial cells.

Structure	Level	Pattern	Image	Note
TS28: cochlea epithelium	Present		5B , 5A	Expression is seen in the cochlear non-sensory epithelium, stria vascularis, Reissner's membrane and auditory nerve fibers; it is not expressed in hair cells.
TS28: cochlear duct	Present		5B , 5A	Expression is seen in the cochlear non-sensory epithelium, stria vascularis, Reissner's membrane and auditory nerve fibers; it is not expressed in hair cells.
TS28: stria vascularis	Present		5B , 5A	Expression is seen in the cochlear non-sensory epithelium, stria vascularis, Reissner's membrane and auditory nerve fibers; it is not expressed in hair cells.
TS28: vestibulocochlear nerve cochlear component	Present		5B , 5A	Expression is seen in the cochlear non-sensory epithelium, stria vascularis, Reissner's membrane and auditory nerve fibers; it is not expressed in hair cells.

Structure	Level	Pattern	Image	Note
TS28: vestibular epithelium	Present	Regionally restricted	5D , 5C	Expression is seen in the epithelium and the supporting cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	5E , 5G , 5F	Expression is seen in the cochlear epithelium and most supporting cell types, including border cells, inner phalangeal cells, pillar cells, Hensen's cells, Deiters' cells and Claudius' cells; it is not seen in the tympanic border cells.

Structure	Level	Pattern	Image	Note
TS23: cochlea	Present		6A'' , 6A' , 6A	Expression is seen in the apical turn.

Structure	Level	Pattern	Image	Note
TS23: cochlea	Present		6B'' , 6B , 6B'	Expression is seen in the middle turn, including in presumptive inner hair cells.

Structure	Level	Pattern	Image	Note
TS23: cochlea	Present		6C , 6C' , 6C''	Expression is seen in the basal turn, including in presumptive inner and outer hair cells.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Present		7A' , 7A'' , 7A	Expression is seen in the apical turn, including in presumptive inner hair cells.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Present		7B , 7B' , 7B''	Expression is seen in the mid-apical turn, including in presumptive inner hair cells.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Present		7C'' , 7C , 7C'	Expression is seen in the mid-basal turn, including in presumptive inner and outer hair cells.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Present		7D' , 7D , 7D''	Expression is seen in the basal turn, although it is not seen in the presumptive inner and outer hair cells.

Structure	Level	Pattern	Image	Note
TS20: inner ear vestibular component	Present		S2A' , S2A , S2A''	Expression is seen in the vestibule, including in presumptive hair cells.