About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:285969 Liu H, et al., SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice. Nucleic Acids Res. 2019 Jun 20;47(11):5670-5683
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6431473
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phosphoH2AX (#05-636; JBW301)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S4j: postnatal day 25 (more )
Note: Wild type. Double labeled: green - H2ax; red - Sycp3 (assay MGI:6431459).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bj green , S4bj merge At pachytene stage, expression is seen on the chromatin of the unsynapsed XY bivalent.
Specimen S4k: postnatal day 25; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: green - H2ax; red - Sycp3 (assay MGI:6431459).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bk green , S4bk merge Unlike in wild type, expression is seen on autosomes of some pachytene-like spermatocytes that had some axial elements synapsed with several parters. This is in addition to the expected expression on the chromatin of the unsynapsed XY bivalent.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory