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Gene Expression Data
Assay Details
Assay
Reference: J:285969 Liu H, et al., SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice. Nucleic Acids Res. 2019 Jun 20;47(11):5670-5683
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6431459
Gene symbol: Sycp3
Gene name: synaptonemal complex protein 3
Antibody: Anti-SCP3 (ab15093)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 4Z wild type: postnatal (more )
Note: Double labeled: red - Sycp3; green - Mlh1 (assay MGI:6431464).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4Z upper Staining is seen in pachytene spermatocytes.
Specimen 4Z mutant: postnatal; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Mlh1 (assay MGI:6431464).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4Z lower Staining is seen in mutant pachytene-like spermatocytes.
Specimen S4j: postnatal day 25 (more )
Note: Wild type. Double labeled: red - Sycp3; green - H2ax (assay MGI:6431473).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bj merge , S4bj red Staining is seen in pachytene spermatocytes.
Specimen S4k: postnatal day 25; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - H2ax (assay MGI:6431473).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bk red , S4bk merge Staining is seen in mutant pachytene-like spermatocytes.
Specimen S6d: embryonic day 17.5 (more )
Note: Wild type. Double labeled: red - Sycp3; green - Mlh1 (assay MGI:6431464).
Structure Level Pattern Image Note
TS25: oocyte Present S6d Staining is seen in pachytene oocytes.
Specimen S6e: embryonic day 17.5; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Mlh1 (assay MGI:6431464).
Structure Level Pattern Image Note
TS25: oocyte Present S6e Staining is seen in mutant pachytene-like oocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory