Antibody:	anti-vGlut3
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS23: cochlea	Absent
TS23: utricle	Strong

Structure	Level	Pattern	Image	Note
TS24: utricle epithelium	Strong	Regionally restricted	1A	Expression is seen in hair cells.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Weak	Regionally restricted	1B	Expression is only detected in cochlear basal turn inner hair cells. It is not seen in the middle or apical turns.

Structure	Level	Pattern	Image	Note
TS26: saccule epithelium	Strong	Regionally restricted	1E	Expression is seen in hair cells.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Weak	Regionally restricted	1G	Expression is seen in basal and middle turn inner hair cells. It was never observed in outer hair cells at this age.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	1H'' , 1H	Expression is seen in the inner hair cells of the basal turn. Some weak expression is seen in the outer hair cells in this turn.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Weak	Regionally restricted	1I , 1I''	Expression is seen in the inner hair cells of the middle turn; it is not seen in the outer hair cells in this turn.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Weak	Regionally restricted	1J'' , 1J	Expression is seen in the inner hair cells of the apical turn; it is not seen in the outer hair cells in this turn.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Absent		1K'' , 1K	There is no expression in the apex.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Strong	Regionally restricted	2C , 2C''	Strong expression is seen in the inner hair cells of the basal turn, weak expression is seen in the outer hair cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Strong	Regionally restricted	2D	There is strong expression in the inner hair cells of the basal turn. There is weak/mosaic expression in the outer hair cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Strong	Regionally restricted	2E	There is strong expression in the inner hair cells of the middle turn. There is weak/mosaic expression in the outer hair cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Strong	Regionally restricted	2F	There is strong expression in the inner hair cells of the apical turn. There is no expression in the outer hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Strong	Regionally restricted	3E' , 3E	Expression is seen in the inner hair cells of the basal turn. No expression is seen in the outer hair cells of this turn.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Strong	Regionally restricted	3F' , 3F	Strong expression is seen in the inner hair cells of the middle turn. Weak/mosaic expression is seen in the outer hair cells of this turn.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Strong	Regionally restricted	3G , 3G'	Strong expression is seen in the inner hair cells of the apical turn. Weak/mosaic expression is seen in the outer hair cells of this turn (although there is more expression than is seen in the middle turn).

Structure	Level	Pattern	Image	Note
TS28: utricle epithelium	Strong	Regionally restricted	3H' , 3H	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Strong	Regionally restricted	3I , 3I''	Strong expression is seen in the inner hair cells, none is seen in the outer hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	4G	Expression is seen in the inner hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	4H	Expression is seen in the inner hair cells at levels comparable to those seen in wild type. (What appears to be differences in expression are due to the angle of the optical section.)

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	6A	Expression is seen in the inner hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	6D	Expression is seen in the inner hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	6G	Expression is seen in the inner hair cells.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present	Regionally restricted	7B'' , 7B	Expression is found in the glia, not the neurons. Expression is seen in the cell body and the terminals of the glia.