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Gene Expression Data
Assay Details
Assay
Reference: J:174215 Chen Y, et al., Plasticity of neural crest-placode interaction in the developing visceral nervous system. Dev Dyn. 2011 Aug;240(8):1880-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6381438
Gene symbol: Phox2b
Gene name: paired-like homeobox 2b
Antibody: anti-Phox2b
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1A: embryonic day 12.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Structure Level Pattern Image Note
TS20: submandibular ganglion Weak Regionally restricted 1A Expression in this mutant reduced to 23.6% of values seen in control embryos.
Specimen 1B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: submandibular ganglion Strong Regionally restricted 1B Expression is in the majority of postganglionic neurons of the ganglion.
Specimen 1C: embryonic day 13.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Structure Level Pattern Image Note
TS21: submandibular ganglion Weak 1C
Specimen 1D: embryonic day 12.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Structure Level Pattern Image Note
TS20: sphenopalatine ganglion Weak Regionally restricted 1D Expression in this mutant reduced to 23.6% of values seen in control embryos.
Specimen 1E: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: sphenopalatine ganglion Strong Regionally restricted 1E Expression is in the majority of postganglionic neurons of the ganglion.
Specimen 1F: embryonic day 13.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Structure Level Pattern Image Note
TS21: sphenopalatine ganglion Weak 1F
Specimen 1G: embryonic day 12.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Structure Level Pattern Image Note
TS20: otic ganglion Weak Regionally restricted 1G Expression in this mutant reduced to 23.6% of values seen in control embryos.
Specimen 1H: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: otic ganglion Strong Regionally restricted 1H Expression is in the majority of postganglionic neurons of the ganglion.
Specimen 1I: embryonic day 13.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Structure Level Pattern Image Note
TS21: otic ganglion Weak 1I
Specimen 2A: embryonic day 10.5 (more )
Note: Double labeled: red - Phox2b; green - Isl1 (assay MGI:6381627).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 2A
TS17: facial ganglion Present 2A
Specimen 2B: embryonic day 10.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Note: Double labeled: red - Phox2b; green - Isl1 (assay MGI:6381627).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 2B The ganglia are fused in this mutant.
TS17: facial ganglion Present 2B The ganglia are fused in this mutant.
Specimen 2C: embryonic day 9.5 (more )
Note: Double labeled: red - Phox2b; green - Isl1 (assay MGI:6381627).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Present 2C
Specimen 2D: embryonic day 9.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Note: Double labeled: red - Phox2b; green - Isl1 (assay MGI:6381627).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Present 2D Ganglia are fused in this mutant.
Specimen 2E: embryonic day 9.5 (more )
Note: Double labeled: red - Phox2b; green - Sox10 (assay MGI:6381628).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Present 2E
Specimen 2F: embryonic day 9.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Note: Double labeled: red - Phox2b; green - Sox10 (assay MGI:6381628).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Present 2F Ganglia are fused in this mutant.
Specimen 3A: embryonic day 12.5 (more )
Note: Double labeled: red - Phox2b; green - Sox10 (assay MGI:6381628).
Structure Level Pattern Image Note
TS20: facial ganglion Present 3A , 3C , 3E
TS20: otic ganglion Present 3A , 3C , 3E
Specimen 3B: embryonic day 12.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Note: Double labeled: red - Phox2b; green - Sox10 (assay MGI:6381628).
Structure Level Pattern Image Note
TS20: otic ganglion Present Regionally restricted 3F , 3B , 3D Due to the altered morphology in this mutant it is unclear whether expression seen are the postganglionic neuron precursors in the rostral sphenopalatine ganglion or in the more ventral otic ganglion.
TS20: sphenopalatine ganglion Present Regionally restricted 3F , 3B , 3D Due to the altered morphology in this mutant it is unclear whether expression seen are the postganglionic neuron precursors in the rostral sphenopalatine ganglion or in the more ventral otic ganglion.
TS20: head mesenchyme derived from neural crest Weak Regionally restricted 3D , 3B , 3F Expression is in a stream of cells from the geniculate ganglion into the area normally occupied by the otic ganglion.
Specimen 3G: embryonic day 11.5 (more )
Note: Double labeled: red - Phox2b; green - Sox10 (assay MGI:6381628).
Structure Level Pattern Image Note
TS19: autonomic ganglion Present Regionally restricted 3G , 3I , 3K Expression is in the sphenopalatine and otic ganglia.
TS19: head mesenchyme derived from neural crest Present Regionally restricted 3I , 3G , 3K Expression is in migrating phenopalatine ganglion precursors.
TS19: facial ganglion Present Regionally restricted 3I , 3G , 3K Expression is in cells migrating out of the ventromedial side.
Specimen 3H: embryonic day 11.5; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Note: Double labeled: red - Phox2b; green - Sox10 (assay MGI:6381628).
Structure Level Pattern Image Note
TS19: autonomic ganglion Present Regionally restricted 3H , 3L , 3J Expression is in the sphenopalatine ganglion and is weak in otic ganglion.
TS19: head mesenchyme derived from neural crest Present Regionally restricted 3J , 3L , 3H Expression is in migrating phenopalatine ganglion precursors.
TS19: facial ganglion Present Regionally restricted 3J , 3L , 3H Expression is in cells migrating out of the ventromedial side.
Specimen 4A: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxa3tm1(cre)Moon/Hoxa3+ (more )
Note: Conditional mutant. Triple labeled: red - Phox2b; blue - Sox10 (assay MGI:6381628); green - Cre reporter.
Structure Level Pattern Image Note
TS20: sphenopalatine ganglion Present 4A
Specimen 4B: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxb1tm6(cre)Mrc/Hoxb1+ (more )
Note: Conditional mutant. Triple labeled: red - Phox2b; blue - Sox10 (assay MGI:6381628); green - Cre reporter.
Structure Level Pattern Image Note
TS20: sphenopalatine ganglion Present 4B
Specimen 4C: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxa3tm1(cre)Moon/Hoxa3+ (more )
Note: Conditional mutant. Triple labeled: red - Phox2b; blue - Sox10 (assay MGI:6381628); green - Cre reporter.
Structure Level Pattern Image Note
TS20: submandibular ganglion Present 4C
Specimen 4D: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxb1tm6(cre)Mrc/Hoxb1+ (more )
Note: Conditional mutant. Triple labeled: red - Phox2b; blue - Sox10 (assay MGI:6381628); green - Cre reporter.
Structure Level Pattern Image Note
TS20: submandibular ganglion Present 4D
Specimen 4E: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxa3tm1(cre)Moon/Hoxa3+ (more )
Note: Conditional mutant. Triple labeled: red - Phox2b; blue - Sox10 (assay MGI:6381628); green - Cre reporter.
Structure Level Pattern Image Note
TS20: facial ganglion Present 4E
TS20: otic ganglion Present 4E
Specimen 4F: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxb1tm6(cre)Mrc/Hoxb1+ (more )
Note: Conditional mutant. Triple labeled: red - Phox2b; blue - Sox10 (assay MGI:6381628); green - Cre reporter.
Structure Level Pattern Image Note
TS20: facial ganglion Present 4F
TS20: otic ganglion Present 4F
Specimen 4G: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxa3tm1(cre)Moon/Hoxa3+ (more )
Note: Conditional mutant. Triple labeled: red - Phox2b; blue - Sox10 (assay MGI:6381628); green - Cre reporter.
Structure Level Pattern Image Note
TS20: otic ganglion Present 4G
Specimen 4H: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Hoxa3tm1(cre)Moon/Hoxa3+, Neurog2tm1Fgu/Neurog2tm1Fgu (more )
Note: Conditional mutant. Triple labeled: red - Phox2b; blue - Sox10 (assay MGI:6381628); green - Cre reporter.
Structure Level Pattern Image Note
TS20: otic ganglion Present 4H
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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