Antibody:	R40.76 (MABT11)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Trace	Regionally restricted	2B	The unpolarized pancreatic epithelium was nearly devoid of expression.

Structure	Level	Pattern	Image	Note
TS20: pancreas epithelium	Present	Regionally restricted	2C	Colocalized expression with Cldn1 was toward the lumen of the pancreatic epithelium in discrete areas.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present	Regionally restricted	2D	Extensive colocalization was observed with Cldn1 in tight junctions.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present	Regionally restricted	2E	Extensive colocalization was observed with Cldn1 in tight junctions.

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Present	Regionally restricted	2F	Extensive colocalization was observed with Cldn1 in tight junctions.

Structure	Level	Pattern	Image	Note
TS26: pancreatic duct	Present	Regionally restricted	3B	Co-expression with Cldn2 was noted in the tight junctions of pancreatic ductal epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present	Regionally restricted	3D	Co-expression with Cldn2 was noted in the tight junctions of pancreatic ductal epithelial cells.

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Trace		4B

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	4C	Colocalized expression with Cldn3 at tight junctions in primitive ducts.

Structure	Level	Pattern	Image	Note
TS23: pancreas epithelium	Present	Regionally restricted	4D	Coexpression with Cldn3 was toward the lumen of the pancreatic ductal network.

Structure	Level	Pattern	Image	Note
TS26: pancreas	Present	Regionally restricted	4E	Coexpression with Cldn3 was detected in ductal epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present		4G	Coexpression with Cldn3 was detected.

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Present		4H	Coexpression with Cldn3 was detected.

Structure	Level	Pattern	Image	Note
TS26: pancreatic duct	Present	Regionally restricted	5A	Expression was detected in tight junctions and co-expressed Cldn5.

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Present	Regionally restricted	5B	Expression was detected in tight junctions and co-expressed Cldn5.

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Trace		6A

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	6B	Expression was detected in tight junctions and colocalized with Cldn7.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	6C	Expression was detected in tight junctions and colocalized with Cldn7.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present	Regionally restricted	6D	Expression was detected in tight junctions and colocalized with Cldn7.

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Present	Regionally restricted	6E	Expression was detected in tight junctions and colocalized with Cldn7.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present	Regionally restricted	6F	Expression was detected in tight junctions and colocalized with low Cldn7 staining.