Antibody:	Claudin-3 antibody (34-1700)
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Present	Regionally restricted	4A	Expression was detected in the lateral membrane of Pdx1-positive progenitors all through the epithelial membrane.

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Present	Regionally restricted	4B	Expression was localized to the lateral membrane of pancreatic cells.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	4C	Expression was detected in the lumen of the pancreas and colocalized with Tjp1.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Strong	Regionally restricted	4D	Expression remained strong toward the lumen of tubular structures in the pancreas.
TS23: pancreas epithelium	Present	Regionally restricted	4D	Extensive colocalization with Tjp1 in tight junctions was noted.

Structure	Level	Pattern	Image	Note
TS26: pancreatic acinus	Present	Regionally restricted	4E	Extensive colocalization with Tjp1 in tight junctions was noted.
TS26: pancreatic duct	Present	Regionally restricted	4E	Extensive colocalization with Tjp1 in tight junctions was noted.

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Present	Regionally restricted	4F	Co-expression with Tjp1 was noted in tight junctions.
TS28: pancreatic duct	Present	Regionally restricted	4F	Co-expression with Tjp1 was noted in tight junctions.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present	Regionally restricted	4G	Co-expression with Tjp1 was noted in tight junctions.

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Present	Regionally restricted	4H	Co-expression with Tjp1 was noted in tight junctions.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Absent