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Gene Expression Data
Assay Details
Assay
Reference: J:175544 Dupuis LE, et al., Altered versican cleavage in ADAMTS5 deficient mice; A novel etiology of myxomatous valve disease. Dev Biol. 2011 Sep 1;357(1):152-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6279802
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: anti-Sox9
Results
Specimen 8A: embryonic day 12.5 (more )
Note: Triple labeled: green - Sox9; blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS20: outflow tract endocardial cushion Strong Widespread 8A Highly expressed in endocardial mesenchyme throughout cushion in both wild type and mutant littermates.
Specimen 8B: embryonic day 12.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Triple labeled: green - Sox9; blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS20: outflow tract endocardial cushion Strong Widespread 8B Highly expressed in endocardial mesenchyme throughout cushion in both wild type and mutant littermates.
Specimen 8C/C': embryonic day 14.5 (more )
Note: Double labeled: green - Sox9; red - propidium iodide.
Structure Level Pattern Image Note
TS22: pulmonary valve Present 8C , 8C' Authors report little or no mesenchymal restriction.
Specimen 8D/D': embryonic day 14.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Double labeled: green - Sox9; red - propidium iodide.
Structure Level Pattern Image Note
TS22: pulmonary valve Present Regionally restricted 8D , 8D' Slight differences in expression at the ventricular aspect of the cusps and subjacent to the endocardium.
Specimen 8E: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: pulmonary valve Weak Regionally restricted 8E Decreased expression in wild type compared to earlier in development. Expression restricted to mesenchymal cells.
Specimen 8F: embryonic day 17.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Structure Level Pattern Image Note
TS25: pulmonary valve Present Regionally restricted 8F Author report significant differences compared to wild type. Expression not down-regulated in cusps, with little or no mesenchymal restriction, reminiscent of valve mesenchyme earlier in development.
Specimen 9S: embryonic day 17.5 (more )
Note: Double labeled: green - Sox9; red - propidium iodide.
Structure Level Pattern Image Note
TS25: pulmonary valve leaflet Present 9S
Specimen 9T: embryonic day 17.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Double labeled: green - Sox9; red - propidium iodide.
Structure Level Pattern Image Note
TS25: pulmonary valve leaflet Present 9T
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory