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Gene Expression Data
Assay Details
Assay
Reference: J:198558 Li W, et al., Tissue-specific venous expression of the EPH family receptor EphB1 in the skin vasculature. Dev Dyn. 2013 Aug;242(8):976-88
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6150925
Gene symbol: Ephb1
Gene name: Eph receptor B1
Antibody: EphB1 Antibody (M-19): sc-9319
Detection system: Secondary antibody
Results
Specimen 1A/B/C: embryonic day 15.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: red - Ephb1; green - Ephb4 (assay MGI:6150916); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: vein Present 1B/B'/B" , 1C/C'/C" , 1A/A'/A" Venous-specific staining for the limb skin vasculature. Overlapped with EphB4-lacZ expression in large-diameter veins, whereas expression was much more clearly detectable in branched middle-diameter veins and venous capillaries than EphB4-lacZ expression.
TS23: lymphatic vessel Absent 1A/A'/A" , 1C/C'/C" , 1B/B'/B"
Specimen 2B/E/H: embryonic day 15.5 (more )
Note: Triple labeled: red - Ephb1; green - Lyve1 (assay MGI:6150932); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: lymphatic vessel Absent 2B , 2H , 2E No expression in the Lyve1+ and Nrp2+ lymphatic vasculature in the limb skin.
TS23: vein Present 2H , 2E , 2B
Specimen 2C/F/I: embryonic day 15.5 (more )
Note: Triple labeled: red - Ephb1; green - Nrp2 (assay MGI:6150937); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: vein Present 2C , 2F , 2I
TS23: lymphatic vessel Absent 2I , 2C , 2F No expression in the Lyve1+ and Nrp2+ lymphatic vasculature in the limb skin.
Specimen 3A/E/I: embryonic day 13.5; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: red - Ephb1; green - Efnb2 (assay MGI:6150924); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS21: vascular plexus Present Regionally restricted 3E , 3A , 3I Expressed in capillary plexus. Some Efnb2-lacZ-expressing vessels co-express Ephb1, but most of Efnb2-lacZ-expressing vessels do not express Ephb1.
Specimen 3B/F/J: embryonic day 14.0; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: red - Ephb1; green - Efnb2 (assay MGI:6150924); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS22: vascular plexus Present Regionally restricted 3B , 3F , 3J Expressed in capillary plexus. Some Efnb2-lacZ-expressing vessels co-express Ephb1, but most of Efnb2-lacZ-expressing vessels do not express Ephb1.
Specimen 3C/G/K: embryonic day 15.5; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: red - Ephb1; green - Efnb2 (assay MGI:6150924); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: arterial blood vessel Ambiguous 3K , 3C , 3G No significant expression in Efnb2-lacZ-expressing dermal arterial vasculature.
TS23: vein Present 3K , 3G , 3C
Specimen 3D/H/L: embryonic day 15.5 (more )
Note: Triple labeled: red - Ephb1; green - Nrp1 (assay MGI:6150938); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: arterial blood vessel Ambiguous 3D , 3L , 3H No significant expression in the Nrp1+ arterial vasculature.
TS23: vein Present 3L , 3H , 3D
Specimen 4A/D/G: embryonic day 13.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: red - Ephb1; green - Ephb4 (assay MGI:6150916); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS21: vascular plexus Present Regionally restricted 4G , 4D , 4A Expressed in capillary plexus.
Specimen 4B/E/H: embryonic day 14.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: red - Ephb1; green - Ephb4 (assay MGI:6150916); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS22: vein Present 4H , 4E , 4B
TS22: arterial blood vessel Present 4B , 4H , 4E Expression was progressively decreased.
Specimen 4C/F/I: embryonic day 15.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: red - Ephb1; green - Ephb4 (assay MGI:6150916); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: vein Present 4F , 4I , 4C
Specimen 4D inset: embryonic day 13.0 (more )
Note: Double labeled: red - Ephb1; blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS21: vascular plexus Present Regionally restricted 4D inset Expressed in capillary plexus.
Specimen 5A/D/G: embryonic day 15.5 (more )
Note: Triple labeled: red - Ephb1; green - Acta2 (assay MGI:6150939); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: venous blood vessel Present 5A , 5D , 5G
TS23: arterial blood vessel Ambiguous 5G , 5D , 5A No significant expression was detectable in a typical arterial vasculature.
Specimen 5B/E/H: embryonic day 15.5 (more )
Note: Triple labeled: red - Ephb1; green - Acta2 (assay MGI:6150939); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: venous blood vessel Present 5E , 5B , 5H
Specimen 5C/F/I: embryonic day 15.5 (more )
Note: Triple labeled: red - Ephb1; green - Acta2 (assay MGI:6150939); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: venous blood vessel Present 5F , 5C , 5I
Specimen 6A/C: postnatal adult (more )
Note: Double labeled: red - Ephb1; blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS28: blood vessel Present 6C , 6A
Specimen 6B/D: postnatal adult (more )
Note: Triple labeled: red - Ephb1; green - Prph (assay MGI:6150940); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS28: blood vessel Present 6D , 6B , 6D' , 6B'
TS28: peripheral nerve Absent 6B , 6D , 6D' , 6B'
Specimen 7A/D/G: postnatal adult; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: red - Ephb1; green - Ephb4 (assay MGI:6150916); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS28: lymphatic vessel Present 7D , 7A , 7G
TS28: venous blood vessel Present 7D , 7A , 7G
Specimen 7B/E/H: postnatal adult; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: red - Ephb1; green - Ephb4 (assay MGI:6150916); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS28: venous blood vessel Present 7B , 7H , 7E
Specimen 7C/F/I: postnatal adult (more )
Note: Triple labeled: red - Ephb1; green - Lyve1 (assay MGI:6150932); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS28: venous blood vessel Present 7F , 7C , 7I
Specimen 8A/D: embryonic day 9.5 (more )
Note: Double labeled: red - Ephb1; blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS15: cardinal vein Present 8D , 8A Expressed in endothelial cells.
TS15: dorsal aorta Present 8D , 8A Expressed in endothelial cells.
TS15: vascular plexus Present Regionally restricted 8D , 8A Expressed in perineural vascular plexus.
Specimen 8B/E: embryonic day 13.5 (more )
Note: Double labeled: red - Ephb1; blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS21: cardinal vein Present 8B , 8E Expressed in endothelial cells.
TS21: dorsal aorta Present 8B , 8E Expressed in endothelial cells.
TS21: lung vascular element Present 8B , 8E Expressed in endothelial cells.
TS21: perineural vascular plexus Present 8B , 8E Expressed in endothelial cells.
Specimen 8C/F: embryonic day 13.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: red - Ephb1; green - Ephb4 (assay MGI:6150916); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS21: cardinal vein Present 8C , 8F Expressed in endothelial cells.
TS21: dorsal aorta Present 8C , 8F Expressed in endothelial cells.
TS21: lung vascular element Present 8C , 8F Expressed in endothelial cells.
TS21: perineural vascular plexus Present 8C , 8F Expressed in endothelial cells.
TS21: lymphatic system Absent 8C , 8F
Specimen 9A/C: embryonic day 8.0; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: red - Ephb1; green - Ephb4 (assay MGI:6150916); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 9A , 9C Expressed in capillary networks.
Specimen 9B/D: embryonic day 9.5 (more )
Note: Triple labeled: red - Ephb1; green - Nrp1 (assay MGI:6150938); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Widespread 9B , 9D Expressed in all yolk sac vasculature including Nrp1+ remodeled arteries, Nrp1- remodeled veins and capillaries.
Specimen 9E/G: embryonic day 15.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: red - Ephb1; green - Ephb4 (assay MGI:6150916); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: lung vascular element Present Regionally restricted 9E , 9G Expressed in Ephb4-lacZ-expressing lung microvasculature and Efnb2-lacZ-expressing large-diameter arteries.
Specimen 9F/H: embryonic day 15.5; Efnb2tm1And/Efnb2+ (more )
Note: Triple labeled: red - Ephb1; green - Efnb2 (assay MGI:6150924); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: lung vascular element Present Regionally restricted 9H , 9F Expressed in Ephb4-lacZ-expressing vessels, which appear to form the majority of lung microvasculature.
TS23: pulmonary artery Present Regionally restricted 9H , 9F Expressed in Efnb2-lacZ-expressing large-diameter arteries.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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